Clinical Virology Manual

"Today, the impact of clinical virology has never been more paramount with the emergence and global expansion of diseases caused by viruses such as Dengue, Chikungunya, Ebola and Zika. The Clinical Virology Manual provides an outstanding comprehensive review of the pathogenesis, epidemiology, clinical impact and diagnosis of viral pathogens. The Manual is an essential diagnostic guide for both experienced and up and coming clinical virologists and laboratorians in general." Christine C. Ginocchio, PhD, MT (ASCP), VP, Global Microbiology Affairs, bioMerieux; VP, Scientific and Medical Affairs, BioFire; Professor, Hofstra North Shore-LIJ School of Medicine, NY

Contributors Author Biographies Preface to the Fifth Edition SECTION I GENERAL TOPICS IN CLINICAL VIROLOGY 1 The Taxonomy, Classification, and Characterization of Medically Important Viruses / 3 STEVEN J. DREWS 2 Quality Assurance and Quality Control in Clinical and Molecular Virology / 27 MATTHEW J. BANKOWSKI 3 Regulatory Compliance / 35 LINOJ SAMUEL 4 Laboratory Safety / 41 K. SUE KEHL 5 Laboratory Design / 51 MATTHEW J. BINNICKER SECTION II LABORATORY PROCEDURES FOR DETECTING VIRUSES 6 Specimen Requirements Selection, Collection, Transport, and Processing / 59 REETI KHARE AND THOMAS E. GRYS 7 Primary Isolation of Viruses / 79 MARIE L. LANDRY AND DIANE LELAND 8 Viral Antigen Detection / 95 DIANE S. LELAND AND RYAN F. RELICH 9 Serologic (Antibody Detection) Methods / 105 DONGXIANG XIA, DEBRA A. WADFORD, CHRISTOPHER P. PREAS, AND DAVID P. SCHNURR 10 Nucleic Acid Extraction in Diagnostic Virology / 117 RAYMOND H. WIDEN AND SUZANE SILBERT 11 Nucleic Acid Amplification by Polymerase Chain Reaction / 129 ANA MARIA CARDENAS AND KEVIN ALBY 12 Isothermal Nucleic Acid Amplification Methods / 137 HARALD H. KESSLER AND EVELYN STELZL 13 Quantitative Molecular Methods / 145 NATALIE N. WHITFIELD AND DONNA M. WOLK 14 Signal Amplification Methods / 167 YUN (WAYNE) WANG 15 DNA Sequencing for Clinical and Public Health Virology: Some Assembly Required / 173 JOANNE BARTKUS 16 Phenotypic and Genotypic Antiviral Susceptibility Testing / 201 MARTHA T. VAN DER BEEK AND ERIC C. J. CLAAS 17 Point-of-Care Diagnostic Virology / 229 JAMES J. DUNN AND LAKSHMI CHANDRAMOHAN 18 Future Technology / 243 ERIN MCELVANIA TEKIPPE AND CAREY-ANN D. BURNHAM SECTION III VIRAL PATHOGENS 19 Respiratory Viruses / 257 CHRISTINE ROBINSON, MICHAEL J. LOEFFELHOLZ, AND BENJAMIN A. PINSKY 20 Enteroviruses and Parechoviruses / 277 M. STEVEN OBERSTE AND MARK A. PALLANSCH 21 Measles, Mumps, and Rubella Viruses / 293 WILLIAM J. BELLINI, JOSEPH P. ICENOGLE, AND CAROLE J. HICKMAN 22 Gastrointestinal Viruses / 311 MICHAEL D. BOWEN 23 Hepatitis A and E Viruses / 329 GILBERTO VAUGHAN AND MICHAEL A. PURDY 24 Hepatitis B and D Viruses / 341 REBECCA T. HORVAT 25 Hepatitis C Virus / 351 MELANIE MALLORY AND DAVID HILLYARD 26 Herpes Simplex Viruses and Varicella Zoster Virus / 363 SCOTT H. JAMES AND MARK N. PRICHARD 27 Cytomegalovirus / 373 PREETI PANCHOLI AND STANLEY I. MARTIN 28 Epstein-Barr Virus / 387 DERRICK CHEN AND BELINDA YEN-LIEBERMAN 29 Human Herpesviruses 6, 7, and 8 / 399 SHEILA C. DOLLARD AND TIMOTHY M. KARNAUCHOW 30 Human Papillomaviruses / 413 SUSAN NOVAK-WEEKLEY AND ROBERT PRETORIUS 31 Human Polyomaviruses / 427 REBECCA J. ROCKETT, MICHAEL D. NISSEN, THEO P. SLOOTS, AND SEWERYN BIALASIEWICZ 32 Parvoviruses / 443 RICHARD S. BULLER 33 Poxviruses / 457 ASHLEY V. KONDAS AND VICTORIA A. OLSON 34 Rabies Virus / 473 ROBERT J. RUDD AND APRIL D. DAVIS 35 Arboviruses / 493 LAURA D. KRAMER, ELIZABETH B. KAUFFMAN, NORMA P. TAVAKOLI 36 Animal-Borne Viruses / 515 GREGORY J. BERRY, MICHAEL J. LOEFFELHOLZ, AND GUSTAVO PALACIOS 37 Human Immunodeficiency Viruses and Human T-lymphotropic Viruses / 527 JOERG SCHUEPBACH 38 Chlamydiae / 545 BARBARAVAN DER POL AND CHARLOTTE A. GAYDOS 39 The Human Virome / 561 MATTHEW C. ROSS, NADIM J. AJAMI, AND JOSEPH F. PETROSINO 40 Human Susceptibility and Response to Viral Diseases / 567 VILLE PELTOLA AND JORMA ILONEN APPENDIXES: REFERENCE VIROLOGY LABORATORIES APPENDIX 1 Reference Virology Laboratory Testing Performed at the Centers for Disease Control / 581 ROBERTA B. CAREY APPENDIX 2 Public Health Laboratory Virology Services / 585 JANE GETCHELL APPENDIX 3 International Reference Laboratories Offering Virology Services / 595 ARIEL I. SUAREZ AND CRISTINA VIDELA INDEX / 607