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Språk: Engelska
Antal sidor: 312
Utgivningsdatum: 2011-04-28
Förlag: Elsevier Science
ISBN: 9780080536873

Molecular Imaging E-bok

Name: Molecular Imaging
Price: 1297.00 SEK
Availability: InStock
Author: Ammasi Periasamy, Richard Day
ISBN: 9780080536873

FRET Microscopy and Spectroscopy

av Ammasi Periasamy, Richard Day

E-bok (PDF, LCP), Engelska, 2011-04-28

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The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

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FLIM Microscopy in Biology and Medicine

Ammasi Periasamy, Robert M Clegg

Detecting Signals at the Single Molecule Level: Pioneering Achievements in Microscopy Recent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopys (FLIM) capacity for contrast and sensitivity that researche...

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Methods in Cellular Imaging

Ammasi Periasamy

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy metho...

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