Biosensor Technologies, Hyperspectral Imaging and Practical Applications
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Köp båda 2 för 2849 krThis book primarily covers the general description of foodborne pathogens and their mechanisms of pathogenesis, control and prevention, and detection strategies, with easy-to-comprehend illustrations. The book is an essential resource for food mic...
Foodborne pathogens continue to cause major public health problems worldwide. These organisms are the leading causes of illness and death in less developed countries, killing approximately 1.8 million people annually. In developed countries, foodb...
Arun K. Bhunia is a Professor of Food Microbiology at Purdue University, USA Moon S. Kim is a research physicist with the Agricultural Research Service, USDA, USA Chris R. Taitt is a research biochemist at the Naval Research Laboratory, Washington DC, USA
* List of contributors* Woodhead Publishing Series in Food Science, Technology and Nutrition*1. High throughput screening strategies and technology platforms for detection of pathogens: an introduction* Abstract*1.1 Introduction*1.2 Current detection strategies*1.3 Why high throughput screening (HTS) is needed*1.4 HTS technologies for foodborne pathogens - present and future trends*2. Sampling and sample preparation for sensor-based detection of pathogens in foods* Abstract*2.1 Introduction*2.2 Key issues in sample preparation: from "Farm to Fork to Physician"*2.3 Challenges in sampling from food matrices and on "bulk" surfaces*2.4 Nonspecific vs. specific methods*2.5 Physical methods*2.6 Chemical and combined methods*2.7 Capture and concentration of whole microbial cells*2.8 The use of cleaning materials in sampling*2.9 Capture and concentration of pathogen DNA from complex food matrices*2.10 Innovations in selective enrichment strategies*2.11 Conclusions* Part One: Biorecognition techniques*3. Antibodies, enzymes, and nucleic acid sensors for high throughput screening of microbes and toxins in food* Abstract*3.1 Introduction*3.2 Conventional methods for bacterial pathogen detection*3.3 Rapid and advanced technologies*3.4 Antibody structure and production*3.5 Polyclonal and monoclonal antibodies for biorecognition*3.6 The identification of recombinant antibodies by phage display technology*3.7 Biopanning of phage display libraries*3.8 Biosensors and antibody immobilization strategies*3.9 Immunosensor-based applications for high throughput pathogen screening*3.10 Multiplexed pathogen detection using antibodies for biorecognition*3.11 Nucleic acid assays*3.12 Microarray-based technologies*3.13 Enzyme-based sensors*3.14 High throughput bacterial toxin detection*3.15 High throughput fungal pathogen and mycotoxin detection*3.16 Marine toxins*3.17 Selected commercial platforms for high throughput detection*3.18 Conclusion*4. Phage technology in high throughput screening for pathogen detection in food* Abstract* Acknowledgments*4.1 Introduction*4.2 Pathogen detection using phage: culture-based methods and phage typing*4.3 Pathogen detection using phage: phage-host adhesion-based methods*4.4 Pathogen detection using phage: biosensors*4.5 Pathogen detection using phage: phage-triggered ion cascade*4.6 Pathogen detection using phage: phage replication and metabolism-based methods*4.7 Pathogen detection using phage: phage lysis-based methods*4.8 Conclusion*5. Mammalian cell-based sensors for high throughput screening for detecting chemical residues, pathogens, and toxins in food* Abstract* Acknowledgments*5.1 Introduction*5.2 The need for novel methods in food control*5.3 Cell-based biosensors for food safety*5.4 Mammalian cell-based biosensors*5.5 Robustness and shelf life of mammalian cell-based biosensors*5.6 Conclusions and future trends* Part Two: Optical transducers and hyperspectral imaging*6. Label-free light-scattering sensors for high throughput screening of microbes in food* Abstract* Acknowledgments*6.1 Introduction*6.2 Elastic light-scattering-based high throughput screening of microorganisms*6.3 Application of BARDOT-based high throughput screening in food safety*6.4 Future trends*7. Vibrational spectroscopy for food quality and safety screening* Abstract* Acknowledgments*7.1 Introduction*7.2 Basic concepts of vibrational spectroscopy*7.3 Applications in food quality*7.4 Applications in food safety*7.5 Hyperspectral imaging for food quality and safety*7.6 Summary and future trends*8. Flow cytometry and pathogen screening in foods* Abstract* Acknowledgments*8.1 Introduction*8.2 Analysis of foods using classical flow cytometry*8.3 Analysis of foods using bead-based detection*8.4 Future trends*8.5 Conclusions*9. Fluorescence-based real-time quantitative polymerase chain reaction (qPCR) technologies for high throughput screening of pathogens* Abstract* Acknowledgments*9.1 Introduction*9.2 Basics of real-tim