Developmental Biology Protocols

"We live in a time of rising expectations. Being a developmental biologist at the turn of the millenium demands expertise in embryological manipulation, viral gene expression, in situ hybridization, videomicroscopy, in vitro assays, transgenic embryo production, cell lineage analysis, PCR techniques, and computer-enhanced imaging technology. Rocky Tuan and Cecilia Lo have done the field (and each scientist within it) a wonderful service by collecting and editing the protocols of the masters of each echnique. More than 100 papers spanning these areas (and more) patiently lead one through each method, giving details on what brand of microscope slides to use, what centrifuge tubes work best, and where to purchase each piece of equipment. Copious notes provide details based on the laboratories' experiences of what works and what doesn't. Developmental Biology Protocols is a library in itself and will be essential for every laboratory of developmental biology. This is the collection to get before your next grant application is due."-Scott Gilbert, Department of Biology, Swarthmore College, Swarthmore, PA "The range of techniques, from viral vectors to imaging, and of organisms, from sea urchins to mammals, is comprehensive yet focused. This work should be an invaluable aid to those interested in all aspects of comparative development. This work is logically organized and presents a great opportunity for not only picking up a technique but also placing it in a logical context. Well done!" -Barbara B. Knowles, Director of Research, Jackson Labs, Bar Harbor, ME "Among similar laboratory manuals Developmental Biology Protocols edited by Tuan and Lo impress by their breadth of coverage, timeliness, and scrupulous attention to detail. . .these protocols will be an invaluable aid to every geneticist and developmental biologist."-Davor Solter, Director of Developmental Biology, Max-Planck Institute, Freiburg, Germany

Developmental Biology Protocols.- Manipulation Of Developmental Gene Expression and Function.- Ectopic Expression in Drosophila.- Clonal Analysis in the Examination of Gene Function in Drosophila.- Application of Antisense Oligodeoxynucleotides in Developing Chick Embryos.- Application of Functional Blocking Antibodies.- Analysis of Gene Expression.- Gene Expression Analyzed by Ribonuclease Protection Assay.- Relative Reverse Transcription-Polymerase Chain Reaction.- Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Multispecific Internal Control.- In Situ PCR Detection of HIV Expression in the Human Placenta.- Gene Expression Analysis by In Situ Hybridization.- Radio-Isotopic In Situ Hybridization on Tissue Sections.- mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry.- Whole Mount In Situ Hybridization to Study Gene Expression During Mouse Development.- Multicolor Whole-Mount In Situ Hybridization.- Methods for Double Detection of Gene Expression.- Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins.- Monoclonal Antibodies in the Analysis of Embryonic Development.- Models of Morphogenesis and Development.- Mesoderm Induction in Xenopus.- Amphibian Organizer Activity.- Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery.- Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration.- The Chimeric Human/Mouse Model of Angiogenesis.- Analysis of Embryonic Vascular Morphogenesis.- Epithelial-Mesenchymal Interactions.- Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patterning.- Palate Development.- In Vitro Models and Analysis of Differentiation and Development.- In Vitro Fertilization.- Trophoblast Differentiation.- Bone Marrow-Derived Mesenchymal Progenitor Cells.- Identification, Characterization, and Differentiation of Human Prostate Cells.- Preparation of Chick Striated Muscle Cultures.- Study of Skeletal Myogenesis in Cultures of Unsegmented Paraxial Mesoderm.- Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Cartilage Maturation.- Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells.- Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of Mesenchymal Chondrogenesis.- Skeletogenesis.- Studying Early Hematopoiesis Using Avian Blastoderm Cultures.- Isolation and Culture of Mouse Germ Cells.- Cadherin-Mediated Cell-Cell Interactions.- Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins.- Microinjection of Fluorescently Labeled ?-Actinin into Living Cells.- Developmental Models Of Diseases.- Pax3 and Vertebrate Development.- Genetic-Engineered Models of Skeletal Diseases I.- Genetic-Engineered Models of Skeletal Diseases II.- Transgenic Mouse Models of Craniofacial Disorders.