Proteomic Profiling (inbunden)
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Format
Inbunden (Hardback)
Språk
Engelska
Antal sidor
592
Utgivningsdatum
2021-01-10
Upplaga
2nd ed. 2021
Förlag
Springer-Verlag New York Inc.
Medarbetare
Posch, Anton (ed.)
Illustrationer
91 Illustrations, color; 40 Illustrations, black and white; XV, 592 p. 131 illus., 91 illus. in colo
Dimensioner
262 x 196 x 33 mm
Vikt
1180 g
Antal komponenter
1
Komponenter
1 Hardback
ISBN
9781071611852
Proteomic Profiling (inbunden)

Proteomic Profiling

Methods and Protocols

Inbunden Engelska, 2021-01-10
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This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational modifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Proteomic Profiling: Methods and Protocols, Second Edition serves as an ideal reference for students of biochemistry, biomedicine, biology, and genomics and will be an invaluable source for the experienced, practicing scientist as well.
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  • 2D PAGE: Sample Preparation and Fractionation

    Anton Posch

    Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enab...

Innehållsförteckning

1. How Modern Mass Spectrometry Can Solve Ancient Questions: A multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi Rudolf Grimm 2. Step-by-Step Sample Preparation of Proteins for Mass Spectrometric Analysis Xinping Li, Thomas Franz, Ilian Atanassov, and Thomas Colby 3. Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis Yi-Han Lin, Rodrigo Vargas Eguez, Isha Vashee, and Yanbao Yu 4. Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry Ximo Zhang 5. Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation Marcella Nunes Melo-Braga, Maria Ibanez-Vea, Katarzyna Kulej, and Martin R. Larsen 6. Phos-Tag Fluorescent Gel Staining for the Quantitative Detection of His- and Asp-Phosphorylated Proteins Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike 7. Polyubiquitin Profile in Down Syndrome and Alzheimer Disease Brain Antonella Tramutola and Marzia Perluigi 8. Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing Jiaqi Wu, Will McElroy, Charles Haitjema, Carsten Luck, and Christopher Heger 9. A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes Alin Rai, Haoyun Fang, Monique Fatmous, Bethany Claridge, Qi Hui Poh, Richard J. Simpson, and David W. Greening 10. Human Plasma Extracellular Vesicle Isolation and Proteomic Characterization for the Optimization of Liquid Biopsy in Multiple Myeloma Antonia Reale, Tiffany Khong, Rong Xu, Maoshan Chen, Sridurga Mithraprabhu, Nicholas Bingham, Andrew Spencer, and David W. Greening 11. Isolation of Extracellular Vesicles for Proteomic Profiling Dongsic Choi, Janusz Rak, and Yong Song Gho 12. Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses Prabal Subedi, Michael Schneider, Michael J. Atkinson, and Soile Tapio 13. Flow Cytometry as an Important Tool in Proteomic Profiling Michael P. Blundell, Sharon L. Sanderson, and Tracey A. Long 14. Improved Immunoprecipitation to Mass Spectrometry Method for Enrichment of Low-Abundant Protein Targets Penny Jensen, Bhavin Patel, Suzanne Smith, Renuka Sabnis, and Barbara Kaboord 15. Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors Jonathan M. Preuss, Ute Burret, and Sabine Vettorazzi 16. Bead-Based Multiplex Immunoassays: Procedures, Tips, and Tricks Candice Cox 17. Immunoaffinity-Based Liquid Chromatography Mass Spectrometric Assay to Accurately Quantify the Protein Concentration of HMGB1 in EDTA Plasma Viktoria Anselm, Andreas Steinhilber, Cornelia Sommersdorf, and Oliver Poetz 18. Recombinant Anti-Idiotypic Antibodies in Ligand Binding Assays for Antibody Drug Development Stefan Harth and Christian Frisch 19. cDNA Display-Mediated Immuno-PCR (cD-IPCR): An Ultrasensitive Immunoassay for Biomolecular Detection Chathuni Jayathilake and Naoto Nemoto 20. Chromatin Immunoprecipitation (ChIP) to Study DNA-Protein Interactions Eliza C. Small, Danielle Maryanski, Kevin J. Harvey, Michael-C. Keogh, and Andrea Johnstone 21. Profiling Protein-DNA Interactions by Chromatin Immunoprecipitation in Arabidopsis Hendry Susila, Zeeshan Nasim, Suhyun Jin, Geummin Youn, Hyewon Jeong, Ji-Yul Jung, and Ji Hoon Ahn 22. Biotin Proximity Labeling for Protein-Protein Interac