OMICS-Based Approaches in Plant Biotechnology (inbunden)
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Inbunden (Hardback)
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John Wiley & Sons Inc
Banerjee, Rintu (ed.), Kumar, Garlapati Vijay (ed.), Kumar, S. P. Jeevan (ed.)
228 x 158 x 25 mm
612 g
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OMICS-Based Approaches in Plant Biotechnology (inbunden)

OMICS-Based Approaches in Plant Biotechnology

Inbunden Engelska, 2019-02-27
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Burgeoning world population, decreased water supply and land resources, coupled with climate change, result in severe stress conditions and a great threat to the global food supply. To meet these challenges, exploring Omics Technologies could lead to improved yields of cereals, tubers and grasses that may ensure food security. Improvement of yields through crop improvement and biotechnological means are the need-of-the-hour, and the current book "OMICS-Based Approaches in Plant Biotechnology", reviews the advanced concepts on breeding strategies, OMICS technologies (genomics, transcriptomics and metabolomics) and bioinformatics that help to glean the potential candidate genes/molecules to address unsolved problems related to plant and agricultural crops. The first six chapters of the book are focused on genomics and cover sequencing, functional genomics with examples on insecticide resistant genes, mutation breeding and miRNA technologies. Recent advances in metabolomics studies are elucidated in the next 3 chapters followed by 5 chapters on bioinformatics and advanced techniques in plant biotechnology and crop breeding. The information contained in the volume will help plant breeders, plant biotechnologists, plant biochemists, agriculture scientists and researchers in using this applied research to focus on better crop breeding and stress adaptation strategies.
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Rintu Banerjee, Ex-MNRE- Chair-Professor, Indian Institute of Technology, Kharagpur has created a niche of her own in the area of Biomass Deconstruction/Biofuel Production/Enzyme Technology. In the process of her innovative development, she was granted 8 Indian, 3 international (US, Japanese and Chinese) patents. She has published more than 180 papers in peer-reviewed national/international journals. Garlapati Vijay Kumar is an Assistant Professor at the Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, India. He has 3 patents, 33 research articles in peer-reviewed journals and 10 book chapters to his credit. His research interest areas are: Bioprocess engineering / industrial biotechnology, deployment of OMICS technologies for crop improvement, fermentation technology, biofuels, and biocatalysis. S.P. Jeevan Kumar is a scientist in ICAR-Indian Institute of Seed Science, Mau, U.P, India. His interests include OMICS technologies for plant biotechnology, crop improvement, seed deterioration mechanisms, genetic purity and bioenergy. He has published 25 papers in peer-reviewed journals and multiple book chapters.


Introduction xiii Part 1: Genomics 1 1 Exploring Genomics Research in the Context of Some Underutilized Legumes-A Review 3 Patrush Lepcha, Pittala Ranjith Kumar and N. Sathyanarayana 1.1 Introduction 3 1.2 Velvet Bean [Mucuna pruriens (L.) DC. var. utilis (Wall. ex Wight)] Baker ex Burck 4 1.3 Psophocarpus tetragonolobus (L.) DC. 7 1.4 Vigna umbellata (Thunb.) Ohwiet. Ohashi 8 1.5 Lablab purpureus (L.) Sweet 9 1.6 Avenues for Future Research 10 1.7 Conclusions 12 Acknowledgments 12 References 12 2 Overview of Insecticidal Genes Used in Crop Improvement Program 19 Neeraj Kumar Dubey, Prashant Kumar Singh, Satyendra Kumar Yadav and Kunwar Deelip Singh 2.1 Introduction 19 2.2 Insect-Resistant Transgenic Model Plant 21 2.3 Insect-Resistant Transgenic Dicot Plants 27 2.4 Insect-Resistant Transgenic Monocot Plants 34 2.5 Working Principle of Insecticidal Genes Used in Transgenic Plant Preparation 39 2.6 Discussion 41 References 42 3 Advances in Crop Improvement: Use of miRNA Technologies for Crop Improvement 55 Clarissa Challam, N. Nandhakumar and Hemant Balasaheb Kardile 3.1 Introduction 56 3.2 Discovery of miRNAs 56 3.3 Evolution and Organization of Plant miRNAs 57 3.4 Identification of Plant miRNAs 58 3.5 miRNA vs. siRNA 59 3.6 Biogenesis of miRNAs and Their Regulatory Action in Plants 60 3.7 Application of miRNA for Crop Improvement 61 3.8 Concluding Remarks 62 References 70 4 Gene Discovery by Forward Genetic Approach in the Era of High-Throughput Sequencing 75 Vivek Thakur and Samart Wanchana 4.1 Introduction 75 4.2 Mutagens Differ for Type and Density of Induced Mutations 76 4.3 High-Throughput Sequencing is Getting Better and Cheaper 77 4.4 Mapping-by-Sequencing 77 4.5 Different Mapping Populations for Specific Need 81 4.6 Effect of Mutagen Type on Mapping 83 4.7 Effect of Bulk Size and Sequencing Coverage on Mapping 83 4.8 Challenges in Variant Calling 85 4.9 Cases Where Genome Sequence is either Unavailable or Highly Diverged 85 4.10 Bioinformatics Tools for Mapping-by-Sequencing Analysis 86 Acknowledgments 87 References 87 5 Functional Genomics of Thermotolerant Plants 91 Nagendra Nath Das 5.1 Introduction 91 5.2 Functional Genomics in Plants 93 5.3 Thermotolerant Plants 94 5.4 Studies on Functional Genomics of Thermotolerant Plants 98 5.5 Concluding Remarks 99 Abbreviations 100 References 100 Part 2: Metabolomics 105 6 A Workflow in Single Cell-Type Metabolomics: From Data Pre-Processing and Statistical Analysis to Biological Insights 107 Biswapriya B. Misra 6.1 Introduction 108 6.2 Methods and Data 109 6.2.1 Source of Data 109 6.2.2 Processing of Raw Mass Spectrometry Data 109 6.2.3 Statistical Analyses 109 6.2.4 Pathway Enrichment and Clustering Analysis 110 6.3 Results 110 6.3.1 Design of the Study and Data Analysis 110 6.3.2 The Guard Cell Metabolomics Dataset 110 6.3.3 Multivariate Analysis for Insights into Data Pre-Processing 113 6.3.4 Effect of Data Normalization Methods 119 6.4 Discussion 122 6.5 Conclusion 124 Conflicts of Interest 124 Acknowledgment 125 References 125 7 Metabolite Profiling and Metabolomics of Plant Systems Using 1H NMR and GC-MS 129 Manu Shree, Maneesh Lingwan and Shyam K. Masakapalli 7.1 Introduction 129 7.2 Materials and Methods 131 7.2.1 1H NMR-Based Metabolite Profiling of Plant Samples 132 Metabolite Extraction 132 1H NMR Spectroscopy 132 Qualitative and Quantitative Analysis of NMR Signals 134 7.2.2 Gas Chromatography-Mass Spectroscopy (GC-MS) Based Metabolite Profiling 134 Sample Preparation 134 GC-MS Data Acquisition 135 GC-MS Data Pretreatment and Metabolite Profiling 136 Validation of Identified Metabolites 136 7.2.3 Multivariate Data Analysis 137 7.3 Selected Applications of Metabolomics and Metabolite Profiling 139 Acknowledgments 140 Competing Interests 140