Proteomic Profiling (inbunden)
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Format
Inbunden (Hardback)
Språk
Engelska
Antal sidor
501
Utgivningsdatum
2015-03-28
Upplaga
2015 ed.
Förlag
Humana Press Inc.
Medarbetare
Posch, Anton (ed.)
Illustratör/Fotograf
16 schwarz-weiße Tabellen 60 schwarz-weiße und 63 farbige Abbildungen Bibliographie
Illustrationer
16 Tables, black and white; 68 Illustrations, color; 51 Illustrations, black and white; XV, 501 p. 1
Dimensioner
254 x 178 x 29 mm
Vikt
1112 g
Antal komponenter
1
Komponenter
1 Hardback
ISBN
9781493925490
Proteomic Profiling (inbunden)

Proteomic Profiling

Methods and Protocols

Inbunden Engelska, 2015-03-28
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This volume presents the latest developments of the main pillars of protein analysis, such as sample preparation, separation and characterization. The book begins by describing basic but important sample preparation protocols. It then goes on to describe more sophisticated procedures on enriching specific protein classes and concludes with detailed descriptions of integrated work-flows for comprehensive protein analysis and characterization. The authors of the individual chapters are renowned protein biochemists who have all set value to provide a detailed representation of their lab work. Throughout the chapters, these authors share important tips and tricks for a successful and reproducible employment of their protocols in other laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomic Profiling: Methods and Protocols is the perfect guide for students of Biochemistry, Biomedicine, Biology, and Genomics and will be an invaluable source for the experienced, practicing scientists.
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Fler böcker av Anton Posch

  • 2D PAGE: Sample Preparation and Fractionation

    Anton Posch

    Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enab...

Övrig information

Bio-Rad Laboratories GmbH, 80939 Munchen, Heidemannstr. 164 Sitz der Gesellschaft: Munchen - Amtsgericht Munchen HRB 7491

Innehållsförteckning

1. Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization Stanley Goldberg 2. Sample Preservation Through Heat Stabilization of Proteins: Principles and Examples Mats Boren 3. Isolating Peripheral Lymphocytes by Density Gradient Centrifugation and Magnetic Cell Sorting Frederic Brosseron, Katrin Marcus, and Caroline May 4. Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling Simon Goeddeke, Jorg Kotzka, and Stefan Lehr 5. Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an in vitro Exercise Model Mika Scheler, Martin Hrabe de Angelis, Hadi Al-Hasani, Hans-Ulrich Haring, Cora Weigert, and Stefan Lehr 6. Urinary Pellet Sample Preparation for Shotgun Proteomic Analysis of Microbial Infection and Host Pathogen Interactions Yanbao Yu and Rembert Pieper 7. A Protocol for the Parallel Isolation of Intact Mitochondria from Rat Liver, Kidney, Heart and Brain Sabine Schulz, Josef Lichtmannegger, Sabine Schmitt, Christin Leitzinger, Carola Eberhagen, Claudia Einer, Julian Kerth, Michaela Aichler, and Hans Zischka 8. Isolation of Mitochondria from Cultured Cells and Liver Tissue Biopsies for Molecular and Biochemical Analyses Sabine Schmitt, Carola Eberhagen, Susanne Weber, Michaela Aichler, and Hans Zischka 9. Dynamic Range Compression with ProteoMinerTM: Principles and Examples Lei Li 10. Qualitative and Quantitative Proteomic Analysis of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Omid Azimzadeh, Michael J. Atkinson, and Soile Tapio 11. Full-Length Protein Extraction Protocols and Gel-Based Downstream Applications in Formalin-Fixed Tissue Proteomics Alessandro Tanca, Sergio Uzzau, and Maria Filippa Addis 12. Enrichment of Low-Abundant Protein Targets by Immunoprecipitation Upstream of Mass Spectrometry Barbara Kaboord, Suzanne Smith, Bhavin Patel, and Scott Meier 13. Principles of Protein Labeling Techniques Christian Obermaier, Anja Griebel, and Reiner Westermeier 14. Isolation of Extracellular Vesicles for Proteomic Profiling Dong-Sic Choi and Yong Song Gho 15. A Protocol for Exosome Isolation and Characterization: Evaluation of Ultracentrifugation, Density-Gradient Separation, and Immunoaffinity Capture Methods David W. Greening, Rong Xu, Hong Ji, Bow J. Tauro, and Richard J. Simpson 16. Chloroplast Isolation and Affinity Chromatography for Enrichment of Low Abundant Proteins in Complex Proteomes Roman G. Bayer, Simon Stael, and Markus Teige 17. Depletion of RuBisCO Protein Using the Protamine Sulfate Precipitation Method Ravi Gupta and Sun Tae Kim 18. Step by Step Preparation of Proteins for Mass Spectrometric Analysis Thomas Franz and Xinping Li 19. Identification of Protein N-Termini Using TMPP or Dimethyl Labeling and Mass Spectrometry Jingjing Deng, Guoan Zhang, Fang-ke Huang, and Thomas A. Neubert 20. Optimization of Cell Lysis and Protein Digestion Protocols for Protein Analysis by LC-MS/MS Dominic Winter, Alireza Dehghani, and Hanno Steen 21. Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation and N-Linked Sialylated Glycosylation Marcella Nunes Melo-Braga, Maria Ibanez-Vea, Martin Rossel Larsen, and Katarzyna Kulej 22. Protein Profiling and Phosphoprotein Analysis by Isoelectric Focusing Giuseppina Maccarrone and Michaela D. Filiou 23. Principles and Examples of Gel-Based Approaches for Phosphoprotein Analysis Birgit Steinberger and Corina Mayrhofer 24. Neutral Phosphate-Affinity SDS-PAGE System for Profiling of Protein Phosphorylation Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike 25. Enrichment and Identification of Bacterial Glycopeptides by Mass Spectrometry Nichollas E. Scott and Stuart J. Cordwell 26. In-Gel Peptide IEF Sample Preparation for LC/MS Analysis Thomas Berkelman, Sricharan Bandhakavi, and Aran Paulus 27. Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Stain-Free Technolo