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Format: Häftad (Paperback / softback)
Språk: Engelska
Antal sidor: 274
Utgivningsdatum: 2016-08-23
Upplaga: Softcover reprint of the original 1st ed. 2016
Förlag: Humana Press Inc.
Medarbetare: Kühn, Ralf (ed.), Wefers, Benedikt (ed.), Wurst, Wolfgang (ed.)
Illustrationer: 50 Illustrations, color; 15 Illustrations, black and white; XII, 274 p. 65 illus., 50 illus. in colo
Dimensioner: 254 x 178 x 15 mm
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Antal komponenter: 1
Komponenter: 1 Paperback / softback
ISBN: 9781493949052

TALENs

Name: TALENs
Price: 1550.00 SEK
Availability: InStock
Author: Ralf Khn, Wolfgang Wurst, Benedikt Wefers
ISBN: 9781493949052

Methods and Protocols

av Ralf Khn, Wolfgang Wurst, Benedikt Wefers

Häftad, Engelska, 2016-08-23

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This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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Gene Knockout Protocols

Ralf Khn, Wolfgang Wurst

Following the completion of the mouse and human genome sequences, a major challengeisthefunctionalcharacterizationofeverymammaliangeneandthedeciph- ing of their molecular interaction network. The mouse offers many advantages for the use of genetic...

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Xanthomonas and the TAL effectors: Nature's Molecular Biologist.- TAL Effector DNA-binding Principles and Specificity.- The Development of TALE Nucleases for Biotechnology.- Online Tools for TALEN Design.- Assembly of Customized TAL Effectors through Advanced ULtiMATE System.- Engineering Customized TALENs Using the Platinum Gate TALEN Kit.- Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors.- A New Approach to Dissect Nuclear Organization; TALE-mediated Genome Visualization (TGV).- TALEN Induced Translocations in Human Cells.- Mutagenesis in Newts; Protocol for Iberian Ribbed Newts.- Targeted Mutagenesis in Bombyx mori Using TALENs.- GeneKnockout by Targeted Mutagenesis in a Hemimetabolous Insect, The TwoSpotted Cricket Gryllus bimaculatus, using TALENs.- Methods for TALEN Evaluation, use, and Mutation Detection in the Mosquito Aedes aegypti.- Methods for TALEN-mediated Genomic Manipulations in Drosophila.- Targeted Mutagenesis in Zebrafish by TALENs.- Mutagenesis in Xenopus and Zebrafish using TALENs.- Genome Editing in Mice using TALE Nucleases.- Genome Editing in Rats using TALE Nucleases.- Designer Nuclease-mediated Generation of Knock out THP1 Cells.