The Surfaceome (inbunden)
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Format
Inbunden (Hardback)
Språk
Engelska
Antal sidor
348
Utgivningsdatum
2017-12-20
Upplaga
1st ed. 2018
Förlag
Humana Press Inc.
Medarbetare
Boheler, Kenneth R. (ed.), Gundry, Rebekah L. (ed.)
Illustratör/Fotograf
Bibliographie 21 schwarz-weiße und 47 farbige Abbildungen
Illustrationer
47 Illustrations, color; 21 Illustrations, black and white; XII, 348 p. 68 illus., 47 illus. in colo
Dimensioner
254 x 178 x 21 mm
Vikt
840 g
Antal komponenter
1
Komponenter
1 Hardback
ISBN
9781493975518

The Surfaceome

Methods and Protocols

Inbunden,  Engelska, 2017-12-20
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This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions totheir respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.
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Innehållsförteckning

Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens.- Shaving Live Bacterial Cells with Proteases for Proteomic Analysis of Surface Proteins.- Methods for Mapping the Extracellular and Membrane Proteome in the Avian Embryo, and Identification of Putative Vascular Targets or Endothelial Genes.- Mass Spectrometry Based Identification of Extracellular Domains of Cell Surface N-Glycoproteins: Defining the Accessible Surfaceome for Immunophenotyping Stem Cells and their Derivatives.- Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and its Proteome from Cells in Suspension.- Proteomic Profiling of Secreted Proteins, Exosomes, and Microvesicles in Cell Culture Conditioned Media.- Cloning, Expression, and Purification of the Glycosylated Transmembrane Protein, Cation-Dependent Mannose 6-phosphate Receptor, from Sf9 Cells Using the Baculovirus System.- Bispecific Antibody Armed T Cells to Target Cancer Cells.- Immunophenotyping of Live Human Pluripotent Stem Cells by Flow Cytometry.- Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4.- NaV Channels: Assaying Biosynthesis, Trafficking, Function.- High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs).- Methods for Evaluation of Vascular Endothelial Cell Function with Transient Receptor Potential (TRP) Channel Drugs.- Methods to Study the Signal Transduction of the Surface Receptor Tyrosine Kinase TrkB in Neurons.- Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma Membranes.- Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells under Flow.- Generation of Induced Pluripotent Stem Cells from Patients with COL3A1 Mutations and Differentiation to Smooth Muscle Cells for ECM-Surfaceome Analyses.- Fabrication and Mechanical Properties Measurements of 3D Microtissues for the Study of Cell-Matrix Interactions.- Discovery of Surface Target Proteins Linking Drugs, Molecular Markers, Gene Regulation, Protein Networks, and Disease by Using a Web-Based Platform Targets-Search.