Methods and Protocols
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Köp båda 2 för 1794 krThis volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches...
This book is concerned with basic molecular biology and how research in this field relates to our understanding of molecular and cellular aspects of cardiac development and growth. The authors point out that molecular biology has provided a wealth...
Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens.- Shaving Live Bacterial Cells with Proteases for Proteomic Analysis of Surface Proteins.- Methods for Mapping the Extracellular and Membrane Proteome in the Avian Embryo, and Identification of Putative Vascular Targets or Endothelial Genes.- Mass Spectrometry Based Identification of Extracellular Domains of Cell Surface N-Glycoproteins: Defining the Accessible Surfaceome for Immunophenotyping Stem Cells and their Derivatives.- Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and its Proteome from Cells in Suspension.- Proteomic Profiling of Secreted Proteins, Exosomes, and Microvesicles in Cell Culture Conditioned Media.- Cloning, Expression, and Purification of the Glycosylated Transmembrane Protein, Cation-Dependent Mannose 6-phosphate Receptor, from Sf9 Cells Using the Baculovirus System.- Bispecific Antibody Armed T Cells to Target Cancer Cells.- Immunophenotyping of Live Human Pluripotent Stem Cells by Flow Cytometry.- Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4.- NaV Channels: Assaying Biosynthesis, Trafficking, Function.- High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs).- Methods for Evaluation of Vascular Endothelial Cell Function with Transient Receptor Potential (TRP) Channel Drugs.- Methods to Study the Signal Transduction of the Surface Receptor Tyrosine Kinase TrkB in Neurons.- Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma Membranes.- Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells under Flow.- Generation of Induced Pluripotent Stem Cells from Patients with COL3A1 Mutations and Differentiation to Smooth Muscle Cells for ECM-Surfaceome Analyses.- Fabrication and Mechanical Properties Measurements of 3D Microtissues for the Study of Cell-Matrix Interactions.- Discovery of Surface Target Proteins Linking Drugs, Molecular Markers, Gene Regulation, Protein Networks, and Disease by Using a Web-Based Platform Targets-Search.