Clinical Applications of PCR (häftad)
Format
Häftad (Paperback / softback)
Språk
Engelska
Antal sidor
191
Utgivningsdatum
2018-03-31
Upplaga
Softcover reprint of the original 3rd ed. 2016
Förlag
Humana Press Inc.
Medarbetare
Singh, Rajesh / Patel, Keyur Pravinchandra
Illustrationer
15 Tables, color; 27 Tables, black and white; 16 Illustrations, color; 9 Illustrations, black and wh
Antal komponenter
1
Komponenter
1 Paperback / softback
ISBN
9781493980307
Clinical Applications of PCR (häftad)

Clinical Applications of PCR

Häftad Engelska, 2018-03-31
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This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routine clinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Clinical Applications of PCR, Third Edition serves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.
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"This multi-author treatise reviews the clinical applications of PCR in various fields of medicine stressing its importance for genetic analysis. It has 16 chapters, each with color illustrations and references. Recommended for researchers in biology and hospital laboratories." (Pediatric Endocrinology Reviews (PER), Vol. 14 (3), March, 2017)

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Innehållsförteckning

1. A Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care Alain R. Thierry 2. COLD-PCR: Applications and Advantages Zhuang Zuo and Kausar J. Jabbar 3. PCR-Based Detection of DNA Copy Number Variation Meenakshi Mehrotra 4. Emulsion PCR: Techniques and Applications Rashmi Kanagal-Shamanna 5. Digital PCR: Principles and Applications Rashmi Kanagal-Shamanna 6. Quantitative PCR for Plasma Epstein-Barr Virus Loads in Cancer Diagnostics Sanam Loghavi 7. High Resolution Melt Curve Analysis in Cancer Mutation Screen Meenakshi Mehrotra and Keyur P. Patel 8. Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutations Khedoudja Nafa, Meera Hameed, and Marie E. Arcila 9. Genotyping of Frequent Mutations in Solid Tumors by PCR-Based Single-Base Extension and MassARRAY Analysis Alberto Paniz Mondolfi and Rajesh R. Singh 10. Microfluidics-Based PCR for Fusion Transcript Detection Hui Chen 11. Polymerase Chain Reaction Diagno sis of Leishmaniasis: A Species-Specific Approach &n bsp; Eglys Gonzalez-Marcano, Hirotomo Kato, Juan Luis Concepcion, Maria Elizabeth Marquez, and Alberto Paniz-Mondolfi 12. Detection of Trypanosoma cruzi by Polymerase Chain Reaction Maria Elizabeth Marquez, Juan Luis Concepcion, Eglys Gonzalez-Marcano, and Alberto Paniz-Mondolfi 13. PCR Techniques in Next-Generation Sequencing Rashmi S. Goswami 14. Single Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics Chi Young Ok, Rajesh R. Singh, and Alaa A. Salim 15. Quantitative Real Time PCR: Recent Advances Charanjeet Singh and Sinchita Roy-Chowdhuri 16. PCR Techniques in Char acterizing DNA Methylation Khalida Wani and Kenneth D. Aldape