Somatic Stem Cells (inbunden)
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Inbunden (Hardback)
Antal sidor
2nd ed. 2018
Humana Press Inc.
Singh, Shree Ram (ed.), Rameshwar, Pranela (ed.)
Bibliographie 7 schwarz-weiße und 53 farbige Abbildungen
52 Illustrations, color; 6 Illustrations, black and white; XI, 284 p. 58 illus., 52 illus. in color.
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1 Hardback
Somatic Stem Cells (inbunden)

Somatic Stem Cells

Methods and Protocols

Inbunden Engelska, 2018-09-09
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This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine.
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Preface... Table of Contents... Contributing Authors... Part I Introduction 1. An Update on the Therapeutic Potential of Stem Cell Pranela Rameshwar, Caitlyn A. Moore, Niloy N. Shah, and Caroline P. Smith Part II Stem Cell Study in Model Organisms and Human 2. Single-Step Plasmid Based Reprogramming of Human Dermal Fibroblasts to Induced Neural Stem Cells Luis Azmitia and Philipp Capetian 3. Isolation and Analysis of Mesenchymal Progenitors of the Adult Hematopoietic Niche Mayra Garcia, Lihong Weng, Xingbin Hu, and Ching-Cheng Chen 4. Identification and Isolation of Mice and Human Hematopoietic Stem Cells Bijender Kumar and Srideshikan Sargur Madabushi 5. Identification and Characterization of Hair Follicle Stem Cells Xiaoyang Wang, Yizhan Xing, and Yuhong Li 6. Methods of Mesenchymal Stem Cell Homing to the Blood-Brain Barrier Peter Conaty, Lauren S. Sherman, Yahaira Naaldijk, Henning Ulrich, Alexandra Stolzing, and Pranela Rameshwar 7. 3D Bioprinting and Stem Cells Caitlyn A. Moore, Niloy N. Shah, Caroline P. Smith, and Pranela Rameshwar 8. Characterization of Gastrospheres Using 3D Coculture System Carlos Antonio do Nascimento Santos, Radovan Borojevic, Luiz Eurico Nasciutti, and Christina MaedaTakiy 9. Markers and Methods to Study Intestinal Stem Cells Nathan Pinto, Beyonce Carrington, Catharine Dietrich, and Shree Ram Singh 10. Quantitative Analysis of Intestinal Stem Cell Dynamics using Microfabricated Cell Culture Arrays Leigh A. Samsa, Ian A. Williamson, and Scott T. Magness 11. Detection, Labeling, and Culture of Lung Stem and Progenitor Cells Ivan Bertoncello, Gianni Carraro, and Jonathan L. McQualter 12. Isolation, Characterization, and Differentiation of Mouse Cardiac Progenitor Cells Santosh Kumar Yadav and Paras Kumar Mishra 13. Isolating and Characterizing Adipose-Derived Stem Cells Guangpeng Liu and Xi Chen 14. Enzyme-Free Isolation of Adipose-Derived Mesenchymal Stem Cell Lauren S. Sherman, Alexandra Conde-Green, Vasanth S. Kotamarti, Edward S. Lee, and Pranela Rameshwar 15. Identification and Characterizations of Annulus Fibrosus-Derived Stem Cells Qianping Guo, Pinghui Zhou, and Bin Li 16. The Maintenance of Tendon Stem/Progenitor Cells in Culture Jianying Zhang and James H-C. Wang 17. Intravital Imaging to Understand Spatiotemporal Regulation of Osteogenesis and Angiogenesis in Cranial Defect Repair and Regeneration Xinping Zhang 18. Beating Heart Cells from Hair Cells Robert M. Hoffman and Yasuyuki Amoh 19. Generation of FLIP and FLIP-FlpE Targeting Vectors for Bi-Allelic Conditional and Reversible Gene Knockouts in Mouse and Human Cells Bon-Kyoung Koo 20. Analytical Platforms and Techniques to Study Stem Cells Metabolism Christine Tang, Kevin Chen, Aleksandar Bajic, William T. Choi, Dodge L. Baluya, and Mirjana Maletic-Savatic