Recombinant Protein Expression in Mammalian Cells (inbunden)
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Inbunden (Hardback)
Antal sidor
1st ed. 2018
Humana Press Inc.
Hacker, David L. (ed.)
68 farbige Tabellen 8 schwarz-weiße und 68 farbige Abbildungen Bibliographie
68 Tables, color; 68 Illustrations, color; 8 Illustrations, black and white; XI, 311 p. 76 illus., 6
266 x 184 x 19 mm
780 g
Antal komponenter
1 Hardback
Recombinant Protein Expression in Mammalian Cells (inbunden)

Recombinant Protein Expression in Mammalian Cells

Methods and Protocols

Inbunden Engelska, 2018-09-22
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This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.
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1. Transient Gene Expression in Suspension HEK293-EBNA1 Cells Denis L'Abbe, Louis Bisson, Christian Gervais, Eric Grazzini and Yves Durocher 2. Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells Joanna Pieprzyk, Samuel Pazicky and Christian Loew 3. PEI-Mediated Transient Gene Expression in CHO Cells Yashas Rajendra 4. Stable Expression by Lentiviral Transduction of Cells Natascha Goedecke, Hansjoerg Hauser and Dagmar Wirth 5. Inducible Protein Production in 293 Cells using the piggyBac Transposon System Iacovos P. Michael and Andras Nagy 6. Recombinant CHO Cell Pool Generation using piggyBac Transposon System Sowmya Balasubramanian 7. Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression Cristina Parola, Derek Mason, Andreas Zingg, Daniel Neumeier and Sai T. Reddy 8. Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor David I. Fisher 9. CHO and HEK293 Cell Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors David L. Hacker, Laurence Durrer and Soraya Quinche 10. Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary Cells in Suspension Dominique Monteil and Jeffrey Kuan 11. Continuous and Integrated Expression and Purification of Recombinant Antibodies Sebastian Vogg, Moritz Wolf and Massimo Morbidelli 12. High Throughput Transfection of HEK293 Cells for Transient Protein Production Tia A. Arena, Peter D. Harms and Athena W. Wong 13. Microfluidic Transfection for High-Throughput Mammalian Protein Expression Kristina Woodruff and Sebastian J. Maerkl 14. Genome Wide High Throughput RNAi Screening for Identification of Genes Involved in Protein Production Sarah Inwood, Michael Betenbaugh, Madhu Lal and Joseph Shiloach 15. Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells Kevin Kellner, Ankur Solanki, Thomas Amann, Nga T Lao and Niall Barron 16. Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells Qiong Wang, Cheng-Yu Chung, Julian N. Rosenberg, Geng Yu and Michael J. Betenbaugh 17. Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV) Daniel Blessing, Nicole Deglon and Bernard L. Schneider 18. Considerations in the Use of Codon Optimization for Recombinant Protein Expression Vincent P. Mauro and Stephen A. Chappell 19. Versatile Cell-free Protein Synthesis Systems based on Chinese Hamster Ovary Cells Lena Thoring and Stefan Kubick