Rheumatoid Arthritis (inbunden)
Inbunden (Hardback)
Antal sidor
1st ed. 2018
Humana Press Inc.
Liu, Shuang (ed.)
Bibliographie 24 schwarz-weiße Abbildungen
34 Illustrations, color; 25 Illustrations, black and white; IX, 253 p. 59 illus., 34 illus. in color
254 x 178 x 16 mm
681 g
Antal komponenter
1 Hardback
Rheumatoid Arthritis (inbunden)

Rheumatoid Arthritis

Methods and Protocols

Inbunden Engelska, 2018-09-23
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This volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study.
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Part I: Animal Models 1. Collagen-induced Arthritis Models Miyoshi Maya and Shuang Liu 2. Human Xenograft Model Shuang Liu 3. Long-term Constant Subcutaneous Drug Administration Shuang Liu and Maya Miyoshi 4. Clinical Scoring of Disease Activity in Animal Models Maya Miyoshi and Shuang Liu 5. Histological Analysis of Arthritic Joints Takeshi Kiyoi 6. Preparation of Joint Extracts Shuang Liu and Erika Takemasa Part II: Therapeutic Approach 7. Production of Immunizing Antigen Proteoliposome Using Cell-free Protein Synthesis System Wei Zhou and Hiroyuki Takeda 8. Re-construction of Protein/Liposome Complex Yasuyuki Suzuki 9. Production of Neutralizing Antibody Erika Takemasa, Shuang Liu, and Hitoshi Hasegawa 10. Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen Hiroyuki Takeda 11. Generation of Specific Aptamers Shuang Liu, Yasuyuki Suzuki, and Makoto Inui 12. Production of Lentiviral Particles Shuang Liu 13. RNA Interference Ex Vivo Shuang Liu 14. Lentiviral-mediated Systemic RNA Interference in vivo Shuang Liu 15. Mesenchymal Stem Cell Engineering Shuang Liu Part III: Evaluation of Immunological Status 16. Screening of Ca2+ Influx in Lymphocytes Erika Takemasa and Shuang Liu 17. Single-cell Ca2+ Imaging Shuang Liu 18. Electrophysiological Methods to Measure Ca2+ Current Shuang Liu and Miyuki Kuno 19. The Functional Assessment of T cells Saho Maruyama 20. Release of Antibodies and Cytokines from B cells Shuang Liu 21. Evaluation of Autoreactive Responses Shaung Liu 22. Bone-resorption Activity in Mature Osteoclasts Takeshi Kyoi 23. Animal Models of Vasculitis Mogi M and Shuang Liu Part IV: Clinical Approach 24. Design an Intervention Study Hitoshi Hasegawa 25. Assessment of Disease Activity, Structural Damage, and Function in Rheumatoid Arthritis Hitoshi Hasegawa