Methods and Protocols
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Köp båda 2 för 2205 krDetermination of the 3D Genome Organization of Bacteria using Hi-C.- Overview of Processing and Analyzing Hi-C Data on Bacteria.- GeF-seqA Simple Procedure for Base Pair Resolution ChIP-seq.- Genomic SELEX Screening of Regulatory Targets of Escherichia coli Transcription Factors.- Modular assembly of Synthetic Secondary Chromosomes.- High-resolution Characterization of DNA/Protein Complexes in Living Bacteria.- Imaging of Transcription and Replication in the Bacterial Chromosome with Multicolor Three-dimensional Superresolution Structured Illumination Microscopy.- Genetic Approaches to Study the Interplay Between Transcription and Nucleoid-associated Proteins in Escherichia coliAtomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organization.- Dynamic Light Scattering of DNA-ligand Complexes.- Microscale Thermophoresis Analysis of Chromatin Interactions.- Quantitative Determination of DNA Bridging Efficiency of Chromatin Proteins.- Approaches for Determining DNA Persistence Length using Atomic Force Microscopy.- Quantitation of DNA Binding Affinity using Tethered Particle Motion.- Observing Bacterial Chromatin Protein-DNA Interactions by Combining DNA Flow-stretching with Single-molecule Imaging.- Unravelling the Biophysical Properties of Chromatin Proteins and DNA using Acoustic Force Spectroscopy.- Unravelling DNA organization with single-molecule Force Spectroscopy using Magnetic Tweezers.- In Vitro Transcription Assay to Quantify Effects of H-NS Filaments on RNA Chain Elongation by RNA Polymerase.- Deciphering 3D Organization Of Chromosomes using Hi-C Data.- Molecular Dynamics Simulation of a Feather-boa Model of Bacterial Chromosome.