De som köpt den här boken har ofta också köpt The Anxious Generation av Jonathan Haidt (inbunden).
Köp båda 2 för 2154 krThis volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL el...
From the reviews of the second edition: "This comprehensive collection of step-by-step protocols in the field of gene targeting and mouse genetics covers such topics as gene modification in embryonic stem cells and mutant phenotype analysis. The book will serve as a valuable resource for researchers, scientists, and students interested in these areas. This is a well-illustrated book . All researchers interested in gene targeting and gene knockout protocols will find this extremely useful." (Omer Iqbal, Doodys Review Service, September, 2009)
Gene Modification in ES Cells.- Overview on Mouse Mutagenesis.- Construction of Gene-Targeting Vectors by Recombineering.- Gene-Trap Vectors and Mutagenesis.- Chromosome Engineering in ES Cells.- Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides.- Generation of shRNA Transgenic Mice.- Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonate.- Stem Cell Manipulation.- Gene Targeting in Mouse Embryonic Stem Cells.- Manipulating Mouse Embryonic Stem Cells.- ES Cell Line Establishment.- Generation of Double-Knockout Embryonic Stem Cells.- Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro.- Cloning of ES Cells and Mice by Nuclear Transfer.- Genetically Engineered Mice.- Isolation, Microinjection and Transfer of Mouse Blastocysts.- Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryos.- VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eight-Cell-Stage Embryos.- Generation of Cre Recombinase-Expressing Transgenic Mice Using Bacterial Artificial Chromosomes.- Inducible Cre Mice.- Creation and Use of a Cre Recombinase Transgenic Database.- Transposon Mutagenesis in Mice.- Lentiviral Transgenesis.- Sperm Cryopreservation and In Vitro Fertilization.- Phenotype Analysis.- Influence of Genetic Background on Genetically Engineered Mouse Phenotypes.- Pathologic Phenotyping of Mutant Mice.- Systemic First-Line Phenotyping.