Low-Cost Methods for Molecular Characterization of Mutant Plants (inbunden)
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Format
Inbunden (Hardback)
Språk
Engelska
Antal sidor
38
Utgivningsdatum
2015-06-09
Upplaga
2015 ed.
Förlag
Springer International Publishing AG
Medarbetare
Hofinger, Bernhard J.
Illustratör/Fotograf
Bibliographie 9 farbige Abbildungen
Illustrationer
3 Illustrations, color; 6 Illustrations, black and white; X, 38 p. 9 illus., 3 illus. in color.
Dimensioner
243 x 160 x 9 mm
Vikt
263 g
Antal komponenter
1
Komponenter
1 Hardback
ISBN
9783319162584

Low-Cost Methods for Molecular Characterization of Mutant Plants

Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

av Bradley J Till, Joanna Jankowicz-Cieslak, Owen A Huynh, Mayada M Beshir, Robert G Laport
Inbunden,  Engelska, 2015-06-09
940
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This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
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Innehållsförteckning

Introduction.- Health and Safety Considerations.- Sample Collection and Storage.- Low-Cost DNA Extraction.- PCR Amplification for Low-Cost Mutation Discovery.- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples.- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants.- Example Data.- Conclusions.

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