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Format: Inbunden (Hardback)
Språk: Engelska
Antal sidor: 336
Utgivningsdatum: 2004-11-01
Upplaga: Parental Advisory ed.
Förlag: Blackwell Verlag GmbH
Illustratör/Fotograf: 74 schwarz-weiße Tabellen 101 schwarz-weiße Abbildungen
Illustrationer: Illustrations
Dimensioner: 255 x 180 x 23 mm
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Antal komponenter: 1
ISBN: 9783527310203

RNA Interference in Practice

Name: RNA Interference in Practice
Price: 1991.00 SEK
Availability: OutOfStock
Author: Ute Schepers
ISBN: 9783527310203

Principles, Basics, and Methods for Gene Silencing in C. elegans, Drosophila, and Mammals

av Ute Schepers

Inbunden, Engelska, 2004-11-01

1991

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This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.

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"I would recommend this book to anyone wishing to establish RNAi-mediated gene silencing in their lab." Comparative & Functional Genomics "Mit diesem Buch stellt die Autorin eine deutliche, gut durchdachte und auf die Praxis ausgerichtete Orientierungshilfe zur Verfgung... Ich wnsche dem Buch, dass es ein schneller Wegbereiter zu erfolgreichen Experimenten wird, hnlich wie die Verbreitung des Protokolls am Anfang der RNAi in C. elegans einen Schub fr neue Erkenntnisse brachte." Nachrichten aus der Chemie "Ein Schwerpunkt des Buches sind die zahlreichen Laborprotokolle zu den verschiedenen Anwendungen der RNAi in den unterschiedlichen Modellsystemen. Diese Verbindung zwischen Experiment und biologischem Hintergrund macht das Buch attraktiv fr Labors, die sich in die RNAi-Techniken einarbeiten wollen." Pipette "Insgesamt ist dieses Buch fr alle, die sich mit RNA-Interferenz befassen, unbedingt zu empfehlen." biologen heute

Ute Schepers studied Chemistry at the Kekul-Institut fr Organische Chemie und Biochemie, University of Bonn (Germany), where she completed her PhD thesis in the laboratory of Prof. Dr. K. Sandhoff in 1997. She then moved to Boston for a post-doctoral fellowship in the laboratory of Tomas Kirchhausen at the Department of Cell Biology, Harvard Medical School. Since her return to Bonn in 2000 she is pursuing her own research projects using a combination of chemical and genetic methods for the further development of RNA interference in mammals. One of her main efforts is the chemical synthesis of novel molecular transporters for therapeutical RNAi application.

Introduction - Mechanism of RNAi - RNAi as a Tool for Functional Genomics RNAi in C.elegans - Practical Application - Target Evaluation - Synthesis and Delivery of dsRNA - Genome-wide Screening RNAi in Drosophila - Practical Application - Synthesis and Delivery of dsRNA - RNAi in Cell Lines RNAi in mammals - Transient RNAi in Cell Culture - Design and Synthesis of siRNAs - Delivery of siRNAs into Cells - Analysis and Validation of siRNAs - RNAi with Hairpin RNAs - Inducible RNAi - RNAi in mice - Screening Methods RNAi Resources - Web Resources - List of Suppliers - Glossary of Terms