Electrospray Ionisation Mass Spectrometry (häftad)

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Format: Häftad (Paperback / softback)
Språk: Engelska
Antal sidor: 64
Utgivningsdatum: 2012-07-14
Förlag: LAP Lambert Academic Publishing
Illustrationer: black & white illustrations
Dimensioner: 229 x 152 x 4 mm
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Antal komponenter: 1
Komponenter: Paperback
ISBN: 9783659184871

Electrospray Ionisation Mass Spectrometry

Name: Electrospray Ionisation Mass Spectrometry
Price: 688.00 SEK
Availability: InStock
Author: Endale Tsegaye Mohammed
ISBN: 9783659184871

Ion Suppression in Electrospray Ionisation Mass Spectrometry

av Endale Tsegaye Mohammed

Häftad, Engelska, 2012-07-14

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Electrospray ionization mass spectrometry (ESI-MS) coupled to reverse-phase high performance liquid chromatography (RP-HPLC) has emerged as one of the most powerful techniques for the analysis of biochemical and pharmaceutical compounds. Trifluoroacetic acid (TFA) is a commonly used additive in HPLC and LC-MS analysis of basic compounds. However, TFA is known to suppress the ESI signals of analytes due to its ability to form strong gas-phase ion pairs with positively-charged analyte ions. This ion-pairing process "masks" the sample positively-charged analyte ions/cations from the ESI-MS electric field by rendering them "neutral". To overcome the signal suppression effect of TFA on basic compounds, we studied a simple and very effective means of minimizing the negative effect of TFA in analysis of aniline, caffeine, arginine and glutamine by addition of acetic acid or propionic acid to analyte solution containing 0.05% TFA. A factor of up to two fold signal enhancement (for arginine and glutamine) and full original signal recovery (for caffeine) were achieved.

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