Plant Genomes: Methods for Genetic and Physical Mapping (häftad)
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Format
Häftad (Paperback / softback)
Språk
Engelska
Antal sidor
250
Utgivningsdatum
2012-10-26
Upplaga
Softcover reprint of the original 1st ed. 1992
Förlag
Springer
Medarbetare
Beckmann, J. S. (ed.), Osborn, T. C. (ed.)
Illustrationer
XI, 250 p.
Dimensioner
234 x 156 x 14 mm
Vikt
381 g
Antal komponenter
1
Komponenter
1 Paperback / softback
ISBN
9789401050777

Plant Genomes: Methods for Genetic and Physical Mapping

av J S Beckmann, T C Osborn
Häftad,  Engelska, 2012-10-26
760
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JACQUES S. BECKMANN & THOMAS C. OSBORN Extraordinary progress has been made in the analyses of the genetic structures of higher eukaryotic genomes. Only ten years elapsed between the initial proposals to use molecular DNA markers for the generation of a complete linkage map of the human genome [5, 17] and the first description of a 10 centimorgan map of one of its chromosomes [22], soon to be followed by others. The availability of molecular DNA markers, henceforth called genomic markers [for a review of their properties see 1, 2, 20], represents a milestone in genetics by providing the capacity for complete genetic coverage of all genomes. It is important to remember that the nature of the DNA polymorphism or of the specific method used to uncover it can be quite different for different marker loci. The genetic variation detected can be a result of a simple point mutation, a DNA insertion/deletion event, or a change in repeat copy number at some hypervariable DNA [11] or micro satellite [21] motif. Currently, the methods of detection can involve use of restriction endonucleases, nucleic acid hybridization, or DNA sequence amplification. Each of these sources of var iation and methods of detection can have utility for different applications. Furthermore, new approaches for the detection of DNA polymorphism are constantly emerging. The primary concern here is that the monitored poly morphism defines a genetic marker 'useful' for the desired application.
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Innehållsförteckning

1. Simple plant DNA isolation procedures.- 2. Methods of Southern blotting and hybridization.- 3. Organelle DNA isolation and RFLP analysis.- 4. Detection of DNA sequence variation for genome analysis.- 5. Pulsed-field gel electrophoresis.- 6. Physical mapping by random clone fingerprint analysis.- 7. Construction and application of a YAC library.- 8. Linkage analysis in human genetics.- 9. Statistical methodologies for mapping and analysis of quantitative trait loci.- 10. Mapping quantitative trait loci using nonsimultaneous and simultaneous estimators and hypothesis tests.- Epilogue: additional reflections.

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