Microfluidics for Single-Cell Analysis (inbunden)
Inbunden (Hardback)
Antal sidor
1st ed. 2019
Springer Verlag, Singapore
Lin, Jin-Ming (ed.)
99 Illustrations, color; 7 Illustrations, black and white; VII, 261 p. 106 illus., 99 illus. in colo
Antal komponenter
1 Hardback
Microfluidics for Single-Cell Analysis (inbunden)

Microfluidics for Single-Cell Analysis

Inbunden Engelska, 2019-09-06
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This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor's laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
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Övrig information

Dr. Jin-Ming Lin graduated from Fuzhou University in 1984 and received a PhD from Tokyo Metropolitan University in 1997. He studied and worked at Showa University and Tokyo Metropolitan University from 1992 to 2002. He was selected for the Chinese Academy of Sciences "100 Talented Researcher Program", and obtained a grant from National Natural Science Foundation of China's Fund for Distinguished Young Scholars in 2001. He was a Professor at the Research Center for Eco-Environmental Sciences, Chinese Academy of Science from 2002 to 2004. He has been a Professor at the Department of Chemistry, Tsinghua University since 2004, and was selected as Cheung Kung Scholars Professor by the Ministry of Education, China in 2008. His current research is focused on cell analysis, microfluidics with mass spectrometry (Chip-MS), and chemiluminescence. He is the author or co-author of 458 original research papers published in international journals, 45 reviews, 4 books and 48 patents. He also services as a contributing editor of Trends in Analytical Chemistry, and associate editor of the Journal of Pharmaceutical Analysis, Luminescence, and Chinese Chemical Letters.


Advances of single-cell analysis on microfluidics.- Microfluidic technology for single-cell capture and isolation.- Single cell culture and analysis on microfluidics.- Droplet microfluidic technology for single cell manipulation.- Droplet-based microfluidics for single cell encapsulation and analysis.- Microfluidics for Single-Cell Genomics.- Microfluidics-mass spectrometry combination systems for single cell analysis.- Micro/nano fluidics-enabled single-cell biochemical analysis.- Microfluidic chip based Live Single-Cell Probes.