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This volume contains protocols specifically designed for studying programmed cell death, and also discusses recent advances in techniques that span broader areas of biology that have been recently used or that have potential to be incorporated int...

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This detailed volume provides a single, valuable reference source for methods that definitively identify and accurately quantify apoptosis. The book begins with common methods utilized to detect and quantitate apoptosis, as well as apoptosis signa...

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This volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein rep...

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With genetic engineering, systems explored in this book now exist allowing for the simple, efficient, and near universally precise genetic manipulation directly in any organism, including the mouse. Herein, these models are applied to a wide field...

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This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a plant chromosome vector system, which allows the int...

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This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques. Chapters details practical tips to overcome any obstac...

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This volume presents detailed protocols for novel strategies and approaches to improve functional understanding of protein N- and C-terminal biology. Protein Terminal Profiling: Methods and Protocols addresses topics such as protease specificity p...

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This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammal...

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This book contains an in-depth treatment of matrix-exponential (ME) distributions and their sub-class of phase-type (PH) distributions. Loosely speaking, an ME distribution is obtained through replacing the intensity parameter in an exponential di...

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This volume presents techniques and recent developments in biochemical approaches to study glutamatergic neurotransmission. This book contains detailed discussions on tracing neuronal pathways, functional or spectroscopic imaging, optogenetic or p...

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This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED micros...

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This book on NeuroAIDS, a collection of chapters written by experts and specialists from around the world, provides a global perspective on HIV and NeuroAIDS in the field, clinic, and laboratory. The chapters address the comorbidity of HIV and oth...

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This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents. The chapters in this book are divided into 5 parts and cover topics such as: advances and obstacl...

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This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologous expr...

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This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers thro...

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This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly s...

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This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture. Prepared for non-specialists as wel...

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This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells f...

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This second edition provides a comprehensive collection of the cutting-edge methods for creating and monitoring transgenic cotton and its application on agricultural and basic research. Chapters detail current status and perspectives of transgenic...

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This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data proc...

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This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived...

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This book reviews recent work on molecular chaperones of the prefoldin family from their discovery in Archaea to the basic clarification of their structure. Even though, there is still a lot to investigate in order to understand the roles and func...

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This book argues that God can be found within the edifice of the scientific understanding of physics, cosmology, biology and philosophy. It is a rewarding read that asks the Big Questions which humans have pondered since the dawn of the modern hum...

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This book explains in a didactic way the basic concepts of spectral mixing, digital numbers and orbital sensors, and then presents the linear modelling technique of spectral mixing and the generation of fractional images. In addition to presenting...

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This book provides insights into the research in the fields of artificial intelligence in combination with Internet of Things (IoT) technologies. Today, the integration of artificial intelligence and IoT technologies is attracting considerable int...

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This book uses mixed methods to extend the concept of wellbeing stocks to refer to dynamic ways of working with others. It addresses metaphors and praxis for weaving together strands of experience. The aim of the wellbeing stocks concept is to ena...

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This book presents the applications of ion-exchange materials in the chemical and food industries. It includes topics related to the application of ion exchange chromatography in water softening, purification and separation of chemicals, separatio...

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This book presents strategies and models for cultural heritage enhancement from a multidisciplinary perspective. It discusses identifying historical, current and possible future models for the revival and enhancement of cultural heritage, taking i...

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This contributed volume presents the outcomes of multidisciplinary studies on the problem of sustainable economic development. The key issues addressed here are economic transformation, crisis management, formation and implementation of industrial...

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This book describes the exciting biology and chemistry of strigolactones. Outgrowth of shoot branches? Development of lateral roots? Interactions with beneficial microorganisms? Avoiding parasitic plants? Responding to drought conditions? These im...

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