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    1. Naturvetenskap och teknik
    2. Matematik och naturvetenskap
    3. Biologi
    4. Cellbiologi

    Cilia: Structure and Motility

    Inbunden, Engelska, 2009

    Del 91 i serien Methods in Cell Biology

    1 979 kr

    Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

    Beskrivning

    Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.

    • Covers protocols for cilia and flagella across systems and species
    • Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time
    • Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

    Produktinformation

    • Utgivningsdatum:2009-12-21
    • Mått:184 x 260 x undefined mm
    • Vikt:740 g
    • Format:Inbunden
    • Språk:Engelska
    • Serie:Methods in Cell Biology
    • Antal sidor:344
    • Förlag:Elsevier Science
    • ISBN:9780123749734

    Utforska kategorier

    • Cellbiologi inom Naturvetenskap och teknik
    • Biologi inom Naturvetenskap och teknik

    Mer om författaren

    Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.

    Innehållsförteckning

    • 1. RNAi approaches to axonemal motor function in trypanosomes (Hill)2. Homologous recombination to knock out dynein genes in Tetrahymena (Gaertig)3. RNAi in Chlamydomonas (Cerutti)4. Planarians as a model system for analysis of ciliary assembly/motility (Rompolas)5. Role of nucleotides in dynein function (Shingyoji)6. Protein modification to probe dynein interactions + purification and identification of crosslinked dynein products (Sakato)7. Analysis of redox-sensitive dynein components (Wakabayashi)8. Purification of dyneins from Chlamydomonas and analysis of dynein-dynein linkers (Kamiya/King)9. Kinase/phosphatase-mediated control of dynein function (Sale)10. Central pair MT complex and associated kinesins (Mitchell)11. Calcium regulation of axonemal function In vitro motility assays (Smith)12. Identification and analysis of dynein regulatory complex components (Porter)13. Isolation and analysis of radial spoke proteins (Yang)14. Purification of dyneins from other model organisms: Ciona, sea urchin, fish (Inaba)15. Rescue of mutant phenotypes by protein electroporation (Kamiya Lab)16. Tubulin/Dynein interactions (Raff)17. Measurement of beat frequency and swimming velocity Cell model reactivation in vitro assays of dynein function (Kamiya)18. Live imaging of ependymal cilia (Lechtreck/Witman)19. Immunogold labeling of axonemal components in situ + flat embedding (Geimer)20. CryoEM approaches to axonemal organization (Nicastro)21. CryoEM of dynein-microtubule complexes (Oda/Kikkawa)22. Bioinformatic approaches to dynein HC classification (Yagi)23. Bioinformatics of non-motor dynein components (Asai)24. Biophysical approaches to dynein mechanism (Sutoh)25. Chlamydomonas flagellar beat analysis (Foster)26. Biophysical measurements of motor function (step size/force production) SAXS of axonemes (Toba/Oiwa)Cryo-Electron Microscope Tomography to Study AxonemalOrganizationDaniela Nicastro2. Electron Microscopic Imaging and Analysis of IsolatedDynein ParticlesAnthony J. Roberts and Stan A. Burgess3. Immunogold Labeling of Flagellar Components In SituStefan Geimer4. Scanning Electron Microscopy to Examine Cells and OrgansJovenal T. SanAgustin, John A. Follit, Gregory Hendricks, and Gregory J. Pazour5. X-ray Fiber Diffraction Studies on Flagellar AxonemesKazuhiro Oiwa, Shinji Kamimura, and Hiroyuki Iwamoto6. Markers for Neuronal CiliaJacqueline S. Domire and Kirk Mykytyn7. Immunofluorescence Staining of Ciliated Respiratory Epithelial CellsHeymut Omran and Niki T. Loges8. Immunoprecipitation to Examine Protein ComplexesGregory J. Pazour9. Tandem Affinity Purification of Ciliopathy-Associated ProteinComplexesKarsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner,Marius Ueffing, and Ronald Roepman10. Crosslinking Methods: Purification and Analysis of Crosslinked Dynein ProductsMiho Sakato11. Analysis of the Ciliary/Flagellar Beating of ChlamydomonasKenneth W. Foster12. Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtiiRitsu Kamiya13. High-Speed Digital Imaging of Ependymal Cilia in the Murine BrainKarl-Ferdinand Lechtreck, Michael J. Sanderson, and George B. Witman14. Observation of Nodal Cilia Movement and Measurement of Nodal FlowYasushi Okada and Nobutaka Hirokawa15. Modification of Mouse Nodal Flow by Applying Artificial FlowShigenori Nonaka16. Measuring Cilium-Induced Ca2þ Increases in Cultured Renal EpitheliaHelle A. Praetorius