Thiol Redox Transitions in Cell Signaling, Part A

ENRIQUE CADENAS, MD, PhD, received his PhD in biochemistry from the University of Buenos Aires, School of Medicine. He is professor of pharmacology and pharmaceutical sciences at the University of Southern California School of Pharmacy and of biochemistry and molecular biology at the University of Southern California Keck School of Medicine, and doctor honoris causa (medicine) at the University of Linköping, Sweden. Cadenas was president of the Society for Free Radical Research International (SFRRI) and is fellow of the Society for Free Radical Biology & Medicine. He served the scientific community by participating on NIH study sections (2002-2006; chair 2006-2008). His research interests include energy and redox metabolism in brain aging and the coordinated inflammatory-metabolic responses in brain and neurodegenerative diseases. Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.

• His-tag switch method for the analysis of S-nitrosylated proteinsIdentification of Protein Thiols in Mitochondrial Oxidative Phosphorylation ComplexesMitochondrial thioredoxin reductase: purification, inhibitor studies, and role in cell signalingassessing cell surface thiol statusInduction of Thioredoxin for Mediating Preconditioning-induced Cellular ResponsesA trans sarcoplasmic reticulum membrane redox sensor in the striated muscle: exploring redox sensitivity of the ryanodine receptor calcium release channelRapid approach for the detection, quantification and discovery of novel sulphenic acid or S-nitrosothiol modified proteins using a biotin-switch methodDirect identification by mass spectrometry of in vivo S-nitrosylated peptides Changing paradigms in theology: From antioxidant defence to redox regulationDetermination of GSNO formation in biological samples by HPLC electrochemical detectionAlteration of thioredoxin reductase 1 levels in elucidating cancer etiologythiol-labeling technology in proteomicsAnalytical methods for the determination of sulfur metabolite concentrations in cell extractsChemical tagging and mass spectrometry-based identification of protein thiols modified by lipid peroxidation-derived a,b-unsaturated aldehydesMeasuring protein thiol redox changes in mitochondriaEngineering of redox domains for monitoring electron transfers spectroscopicallyApproaches to detection of cysteine sulfenic acids in proteins using dimedone-based chemical probesEvaluation of conditions affecting degree of sulfenic acid labeling in redox-sensitive proteinsColorimetric and spectrophotometric assays of sulfiredoxinQuantifying disulfides in specific proteinsA simple method of detecting oxidatively-modified proteinsRole of Glutathione Conjugates in Cell SignalingRegulation of Protein Function by Sulfinic Acid FormationMass spectrometry approaches for the study of the oxidation state of protein cysteine residuesassays of protein tyrosine phosphatase oxidationoxidation state of cellular 2-Cys peroxiredoxins (reduced thiol, disulfide and hyperoxidised) by non-reducing SDS-PAGE and immunoblotting