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This volume discusses the current models and latest methodologies used to explore key questions in transfusion medicine. The chapters in this book cover topics such as mouse models of platelet alloimmunization refractoriness and immune thrombocytopenia; analysis of biotinylated red cell blood cells following transfusion; impact of intravascular hemolysis on erythropoiesis in sickle cell disease; western blot detection of blood group A and B antigens; and the role of marginal zone B cells in FVIII inhibitor development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and comprehensive, Methods in Transfusion Medicine is a valuable resource for all researchers interested in learning more about this field and leading us closer to optimal transfusion practice.