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12 produkter
12 produkter
2 542 kr
Skickas inom 10-15 vardagar
The writing of this book is based on: 1. earlier experience writing textbooks for biology students with a university-level background in biology and biochemistry with many (>200) ?gures in these books (in Hungarian), 2. and on the necessity to present university lectures as power point presentations to catch the interest of s- dents. The author realizes that young readers who were grown up in an information society are relactant to read too much unless they have to take exams. Even then they prefer books which contain illustrations for a better understanding. In collaboration with colleagues, referees and members of the publishing staff an extensive set of photographs and illustrations were collected to provide a graphic follow-up to the text. Further aids for the student, instructor and the curious reader are provided by summaries, extensive sets of readings and references for each chapter, a glossary of the terms, list of abbreviations and a DVD with a red-blue eyeglass to visualize three dimensional chromatin structures. The reader could ask: Why another book on apoptosis? The answer to the question is related to the de?nition of the process. The term apoptosis has been introduced to describe typical morphological changes leading to controlled se- destruction of cells. The ?rst demonstrated biochemical feature of this type of cell death was internucleosomal fragmentation, which was occasionally preceded by the generation of large DNA fragments.
1 189 kr
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To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.
1 619 kr
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This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in Cell Cycle Synchronization: Methods and Protocols, Second Edition cover subjects such as: physical fractionations including centrifugal elutriation of healthy and apoptotic cells, and nuclei of mammalian cells; large scale mitotic cell synchronization; chromosome formation during fertilization in eggs; synchronization of unicellular organisms; hematopoietic stem cells used to improve the engraftment in transplantation; and cell cycle control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and comprehensive, Cell Cycle Synchronization: Methods and Protocols, Second Edition is a valuable resource for PhD students and postdoctoral fellows, and researchers interested in general science, pharmacy, medicine and public health, computer science, and life sciences. Specialists and professionals in cell biology, genetics, molecular biology, biochemistry, and pharmacology will also find this book useful.
1 189 kr
Skickas inom 10-15 vardagar
This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques.
1 619 kr
Skickas inom 10-15 vardagar
This Methods in Molecular Biology(TM) volume offers detailed protocols, with theoretical background, and step-by-step instructions on implementing synchronization, as well as current techniques for the enhanced study of regulatory mechanisms in cell cycle events.
1 081 kr
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This book dealswith biological membranes, focuses on permeabilization and pays particularattention to reversible permeabilization to maintain the viability andphysiological conditions of the cells.Selectivepermeability of biological membranes also known as semipermeability, partialpermeability or differential permeability allows molecules to diffuse, pass bypassive and active or by other types of transport processes mediated byproteins. The firstchapter of the book deals with the composition of biological membranes,characterizes cellular membranes of prokaryotic, eukaryotic cells, membranes ofcellular organelles and the function of biological membranes. The second chapter provides an overview of bilayerpermeability, selectivity of permeabilization and cellular transportprocesses. Chapter3 overviews different cell manipulations that aim to make cells permeable whilemaintaining not only the structural but also the functional integrity of cells.The last chapter deals with applications, particularly withreversible permeabilization to study macromolecular (DNA, RNA, poly-ADP ribose)biosynthetic processes, replication, gene expression, visualization ofreplicons, intermediates of chromosome condensation, genotoxic chromatinchanges, upon treatment with heavy metals and different types of irradiation. The interdisciplinaryaspects of the book contribute to the understanding of the structure of nucleicacids, replicative intermediates, Okazaki fragments, RNA primer mechanism,subphases of replication and repair synthesis, replicons, gene expression,chromosome condensation generated a wealth of information that will attract awide readership. The natural audience engaged in DNA research, includinggenetics, cell and molecular biology, chemistry, biochemistry, medicine,pharmacy will find essential material in the book.
1 081 kr
Skickas inom 10-15 vardagar
This book dealswith biological membranes, focuses on permeabilization and pays particularattention to reversible permeabilization to maintain the viability andphysiological conditions of the cells.Selectivepermeability of biological membranes also known as semipermeability, partialpermeability or differential permeability allows molecules to diffuse, pass bypassive and active or by other types of transport processes mediated byproteins. The firstchapter of the book deals with the composition of biological membranes,characterizes cellular membranes of prokaryotic, eukaryotic cells, membranes ofcellular organelles and the function of biological membranes. The second chapter provides an overview of bilayerpermeability, selectivity of permeabilization and cellular transportprocesses. Chapter3 overviews different cell manipulations that aim to make cells permeable whilemaintaining not only the structural but also the functional integrity of cells.The last chapter deals with applications, particularly withreversible permeabilization to study macromolecular (DNA, RNA, poly-ADP ribose)biosynthetic processes, replication, gene expression, visualization ofreplicons, intermediates of chromosome condensation, genotoxic chromatinchanges, upon treatment with heavy metals and different types of irradiation. The interdisciplinaryaspects of the book contribute to the understanding of the structure of nucleicacids, replicative intermediates, Okazaki fragments, RNA primer mechanism,subphases of replication and repair synthesis, replicons, gene expression,chromosome condensation generated a wealth of information that will attract awide readership. The natural audience engaged in DNA research, includinggenetics, cell and molecular biology, chemistry, biochemistry, medicine,pharmacy will find essential material in the book.
2 220 kr
Skickas inom 10-15 vardagar
The term "heavy metals" is used as a group name of toxic metals and metalloids (semimetals) causing contaminations and ecotoxicity. In strict chemical sense the density of heavy metals is higher than 5 g/cm3. From biological point of view as microelements they can be divided into two major groups. a. For their physiological function organisms and cells require essential microelements such as iron, chromium (III), cobalt, copper, manganese, molidenium, zinc. b. The other group of heavy metals is toxic to the health or environment. Of highest concern are the emissions of As, Cd, Co, Cu, Hg, Mn, Ni, Pb, Sn, Tl. The toxicity of heavy metals is well known at organismal level, while less attention has been paid to their cellular effects. This book describes the toxicity of heavy metals on microorganisms, yeast, plant and animal cells. Other chapters of the book deal with their genotoxic, mutagenic and carcinogenic effects. The toxicity of several metals touch upon the aspects of environmental hazard, ecosystems and human health.Among the cellular responses of heavy metals irregularities in cellular mecha nisms such as gene expression, protein folding, stress signalling pathways are among the most important ones. The final chapters deal with biosensors and removal of heavy metals. As everybody is eating, drinking and exposed to heavy metals on a daily basis, the spirit of the book will attract a wide audience.
1 667 kr
Skickas inom 10-15 vardagar
Homeostasis. The health of an organism is influenced by external and internal changes that may lead to the loss of homeostasis. Under healthy conditions organisms compensate these changes. If compensation fails disease ensues. Attention will be paid to lifestyle, environmental changes, genetic makeup and health system. It will be answered how lifestyle, environment, genetic makeup and social conditions help to maintain or upset the biological balance and lead to cancer.Tumor formation. To understand this process the transfer of intracellular and the pathways of extracellular information (signal transduction) will be reviewed briefly. Loss of cellular balance may lead to cell death (.e.g. apoptosis) or to rapid cell growth of cells leading to tumor formation.Metastasis. Animal tumor models serve to understand the spread of the primary tumor cells to distant locations of the organism. Different types of tumors and metastases will be reviewed.
2 220 kr
Skickas inom 10-15 vardagar
The term “heavy metals” is used as a group name of toxic metals and metalloids (semimetals) causing contaminations and ecotoxicity. In strict chemical sense the density of heavy metals is higher than 5 g/cm3. From biological point of view as microelements they can be divided into two major groups. a. For their physiological function organisms and cells require essential microelements such as iron, chromium (III), cobalt, copper, manganese, molidenium, zinc. b. The other group of heavy metals is toxic to the health or environment. Of highest concern are the emissions of As, Cd, Co, Cu, Hg, Mn, Ni, Pb, Sn, Tl. The toxicity of heavy metals is well known at organizational level, while less attention has been paid to their cellular effects. This book describes the toxicity of heavy metals on microorganisms, yeast, plant and animal cells. Other chapters of the book deal with their genotoxic, mutagenic and carcinogenic effects. The toxicity of several metals touch upon the aspects of environmental hazard, ecosystems and human health. Among the cellular responses of heavy metals irregularities in cellular mechanisms such as gene expression, protein folding, stress signaling pathways are among the most important ones. The final chapters deal with biosensors and removal of heavy metals. As everybody is eating, drinking and exposed to heavy metals on a daily basis, the spirit of the book will attract a wide audience.
2 886 kr
Skickas inom 5-8 vardagar
The writing of this book is based on: 1. earlier experience writing textbooks for biology students with a university-level background in biology and biochemistry with many (>200) ?gures in these books (in Hungarian), 2. and on the necessity to present university lectures as power point presentations to catch the interest of s- dents. The author realizes that young readers who were grown up in an information society are relactant to read too much unless they have to take exams. Even then they prefer books which contain illustrations for a better understanding. In collaboration with colleagues, referees and members of the publishing staff an extensive set of photographs and illustrations were collected to provide a graphic follow-up to the text. Further aids for the student, instructor and the curious reader are provided by summaries, extensive sets of readings and references for each chapter, a glossary of the terms, list of abbreviations and a DVD with a red-blue eyeglass to visualize three dimensional chromatin structures. The reader could ask: Why another book on apoptosis? The answer to the question is related to the de?nition of the process. The term apoptosis has been introduced to describe typical morphological changes leading to controlled se- destruction of cells. The ?rst demonstrated biochemical feature of this type of cell death was internucleosomal fragmentation, which was occasionally preceded by the generation of large DNA fragments.
1 667 kr
Skickas inom 10-15 vardagar
Homeostasis. The health of an organism is influenced by external and internal changes that may lead to the loss of homeostasis. Under healthy conditions organisms compensate these changes. If compensation fails disease ensues. Attention will be paid to lifestyle, environmental changes, genetic makeup and health system. It will be answered how lifestyle, environment, genetic makeup and social conditions help to maintain or upset the biological balance and lead to cancer.Tumor formation. To understand this process the transfer of intracellular and the pathways of extracellular information (signal transduction) will be reviewed briefly. Loss of cellular balance may lead to cell death (.e.g. apoptosis) or to rapid cell growth of cells leading to tumor formation.Metastasis. Animal tumor models serve to understand the spread of the primary tumor cells to distant locations of the organism. Different types of tumors and metastases will be reviewed.