ESACT Proceedings – serie

This book captures, in the form of concise papers of limited length, the essence of the latest development in those fields of animal cell technology. We hope that it will become a useful resource of the most up to date information in Animal Cell Technology, at least until the next meeting in May 2003 in Spain. Elisabeth Lindner-Olsson Nathalie Chatzissavidou Elke Lullau Acknowledgements This meeting would not have been possible without the help of a large number of people, some of whom you will find mentioned below. In addition to the Organizing Committee and the Scientific Committee, Mrs Eva Ljung- kvist and Mrs Ewa Engstrom of SKD Konferensservice have been most important to us. They have had to bear with us, scientists with specific demands for everything. Mrs Ewa Engstrom has also, together with Dr Bjorn Lundgren, organized the trade fare and the fund raising. Mr Henrik Levin at SKD has coordinated the distribution of all confirmation letters for oral presentations and posters as well as the coordination of the manuscripts for the proceedings. We would also like to thank Micael Worbin at TylOsand for local coordination.We have just had a stimulating, pleasant and enriching ESACT 2001 meeting. Your participation was a success and we feel privileged and honored that you decided to come to TylOsand.

The 18th ESACT meeting was celebrated in Granada (Spain) in May 2003, and was entitled "Animal Cell Technology Meets Genomics", in order to reflect that the emerging technologies in the area of genomics, proteomics and other "-omics"-type disciplines will provide key technological assets to increase knowledge and open new horizons in animal cell technology. During the meeting a variety of top-class emerging technologies were presented together with the lastest advances in more mature industrial areas. The meeting was opened by a first session devoted to the understanding of basic cellular mechanisms, and four sessions focused on applied aspects of animal cell technology: Cell-based therapies and gene-based therapies, target discovery and biopharmaceuticals. The Granada Meeting has also seen a special focus on forefront industrial case studies. The spirit and scientific excellence of the 18th ESACT meeting is now reflected in different chapters of the book. The book presents, in form of short papers, a high number of the contributions to the meeting, and has been prepared with the aim to provide a relevant reference of the current research efforts in Animal Cell Technology.

The 19th ESACT meeting was to highlight the novel capabilities of the industry to move the products towards the clinic and was attended by a wide range of workers in the industry and for many it was their first ESACT meeting. The meeting was started with a session on Transcription to Secretion with a notable set of presentations on the emerging issues. The other sessions that followed Therapeutic Cell Engineering, Gene Medicine, Cells to Tissue, Protein products and Process Technology guided the delegates through the advances made for the progression of the biotechnology towards the industrial application of the products from cells. The meeting was supported by some exceptional invited speakers from around the world whose contributions complemented the emerging technologies and the changes being made at the industrial end of the ESACT spectrum. The proceedings here include the short papers adding the knowledge of the previous meetings and provide a reference for the researcher entering, or continuing in the field of Animal Cell Technology.

Regeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- nization. Typically, they need to be biodegradable, showing good cell atta- ment and proliferation and they should possess appropriate mechanical properties (Kim et al. , 2000). Synthetic polymers ful ll most of these requirements but lack cell-adhesion peptides on their surface to enhance cell attachment. Ce- adhesion peptides are present in ECM proteins like collagen and elastin. Thus a synthetic polymer coated with ECM proteins would result in a scaffold that mimics the natural cellular environment with enhanced cell attachment and p- liferation. The new bioactive scaffold will be made by combining a synthetic polymer coated with a layer of recombinant ECM proteins produced by CHO cells. The rst step consists of identifying polymers that give best results in terms of CHO cell attachment and growth. Classical techniques to determine biomass are inappropriate to evaluate 3-D structures. Thus a screening system based on stable GFP expressing CHO cells was used to compare the different scaffolds. Simple uorescent measurement after cell lysis allows determining cell attachment and p- liferation on synthetic polymers. Finally CHO cells producing human recombinant collagen I and elastin were generated. We showed that both proteins are expressed and secreted by CHO DG44 cells. 2 Materials and Methods 2.

The 21st ESACT conference was held in the beautiful surroundings of the CityWest Hotel resort in Dublin, Ireland. For the first time in ESACT history the number of participants exceeded 900: a sign of the ever increasing importance of this area. The conference commenced on Sunday June 5th with two sets of parallel workshops on the subjects listed below. An additional workshop was held on Monday lunchtime of the conferenceProcess Analytical Technology (PAT), Quality by Design (QbD) and other recent regulatory developments.2.      Innovative media products for the 21st century biopharmaceutical industry.3.      The impact of high titre media feed-streams on monoclonal antibody purification.4.      Advances in genomics and proteomics.5.      Stem Cell Technology: new developments and clinical applications.

The 21st ESACT conference was held in the beautiful surroundings of the CityWest Hotel resort in Dublin, Ireland. For the first time in ESACT history the number of participants exceeded 900: a sign of the ever increasing importance of this area. The conference commenced on Sunday June 5th with two sets of parallel workshops on the subjects listed below. An additional workshop was held on Monday lunchtime of the conferenceProcess Analytical Technology (PAT), Quality by Design (QbD) and other recent regulatory developments.2.      Innovative media products for the 21st century biopharmaceutical industry.3.      The impact of high titre media feed-streams on monoclonal antibody purification.4.      Advances in genomics and proteomics.5.      Stem Cell Technology: new developments and clinical applications.

This book captures, in the form of concise papers of limited length, the essence of the latest development in those fields of animal cell technology. We hope that it will become a useful resource of the most up to date information in Animal Cell Technology, at least until the next meeting in May 2003 in Spain. Elisabeth Lindner-Olsson Nathalie Chatzissavidou Elke Lullau Acknowledgements This meeting would not have been possible without the help of a large number of people, some of whom you will find mentioned below. In addition to the Organizing Committee and the Scientific Committee, Mrs Eva Ljung- kvist and Mrs Ewa Engstrom of SKD Konferensservice have been most important to us. They have had to bear with us, scientists with specific demands for everything. Mrs Ewa Engstrom has also, together with Dr Bjorn Lundgren, organized the trade fare and the fund raising. Mr Henrik Levin at SKD has coordinated the distribution of all confirmation letters for oral presentations and posters as well as the coordination of the manuscripts for the proceedings. We would also like to thank Micael Worbin at TylOsand for local coordination.We have just had a stimulating, pleasant and enriching ESACT 2001 meeting. Your participation was a success and we feel privileged and honored that you decided to come to TylOsand.

Regeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- nization. Typically, they need to be biodegradable, showing good cell atta- ment and proliferation and they should possess appropriate mechanical properties (Kim et al. , 2000). Synthetic polymers ful ll most of these requirements but lack cell-adhesion peptides on their surface to enhance cell attachment. Ce- adhesion peptides are present in ECM proteins like collagen and elastin. Thus a synthetic polymer coated with ECM proteins would result in a scaffold that mimics the natural cellular environment with enhanced cell attachment and p- liferation. The new bioactive scaffold will be made by combining a synthetic polymer coated with a layer of recombinant ECM proteins produced by CHO cells. The rst step consists of identifying polymers that give best results in terms of CHO cell attachment and growth. Classical techniques to determine biomass are inappropriate to evaluate 3-D structures. Thus a screening system based on stable GFP expressing CHO cells was used to compare the different scaffolds. Simple uorescent measurement after cell lysis allows determining cell attachment and p- liferation on synthetic polymers. Finally CHO cells producing human recombinant collagen I and elastin were generated. We showed that both proteins are expressed and secreted by CHO DG44 cells. 2 Materials and Methods 2.