This volume discusses the latest advancements on approaches used to characterize C. difficile physiology, including new methods for genetic modifications and novel models for studying host-pathogen interactions. The chapters in this book cover various topics such as FASTQ; CRISPR-AsCas12a and dAsCas 12a-mediated gene knockout and knockdown in Clostridioides difficile; preparation of spores with 70:30 method; measuring C. difficile adhesion to mucosal surfaces; and isolating C. difficile from animal samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Clostridioides difficile: Methods and Protocols is a valuable resource for researchers interested in learning more about this field. Gaining a deeper understanding of C. difficile physiology is crucial for developing new therapies for treatment and predicting the future evolution of this important clinical pathogen.
