Irit Sagi – författare

This second edition volume expands on the previous edition with a discussion of the latest methods used to explore collagen biology from molecular synthesis and folding to biomechanical analysis and tissue engineering applications. The chapters in this book are organized into four parts: Part One introduces strategies for manipulating collagen at the molecular level, including gene targeting, mutagenesis, and functional assays. Part Two focuses on cellular and biochemical aspects of collagen biology, featuring protocols to study the effects of hypoxia, assess triple helix stability, and investigate ECM-rich environments using proteomics and mass spectrometry. Part Three covers structural and imaging techniques such as atomic force microscopy and advanced visualization of collagen suprastructures, and Part Four looks at translational and biomedical research, providing protocols for constructing 3D ECM scaffolds, engineering collagen-based biomaterials, and modeling tumor microenvironments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Collagen: Methods and Protocols, Second Edition is a valuable resource for researchers in cell biology, biochemistry, tissue engineering, and translational medicine, and will support the development of novel strategies to explore and manipulate the power of collagen.

Discussing recent advances in the field of matrix metalloproteinase (MMP) research from a multidisciplinary perspective, Matrix Metalloproteinase Biologyis a collection of chapters written by leaders in the field of MMPs.  The book focuses on the challenges of understanding the mechanisms substrate degradation by MMPs, as well as how these enzymes are able to degrade large, highly ordered substrates such as collagen. All topics addressed are considered in relation to disease progression including roles in cancer metastasis, rheumatoid arthritis and other inflammatory diseases. The text first provides an overview of MMPs, focusing on the history, the development and failures of small molecule inhibitors in clinical trials, and work with TIMPS, the endogenous inhibitors of MMPs. These introductory chapters establish the foundation for later discussion of the recent progress on the design of different types of inhibitors, including novel antibody based therapeutics. The following section emphasizes research using novel methods to further the study of the MMPs. The third and final section focuses on in vivo research, particularly with respect to cancer models, degradation of the extracellular matrix, and MMP involvement in other disease states. Written and edited by leaders in the field, Matrix Metalloproteinase Biology addresses the rapidly growth in MMP research, and will be an invaluable resource to advanced students and researchers studying cell and molecular biology.