Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention, Volume 2

Inbunden, Engelska, 2015

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Beskrivning

Laboratory Techniques in Rabies Diagnosis, Research and Prevention provides a basic understanding of the current trends in rabies. It establishes a new facility for rabies surveillance, vaccine and antibody manufacturing. It offers clarity about the choice of laboratory methods for diagnosis and virus typing, of systems for producing monoclonal and polyclonal antibodies and of methods for testing potency of vaccines and antibodies.The book covers advancements in the classical methods described as well as recent methods and approaches pertaining to rabies diagnosis and research.

Supplies techniques pertaining to rabies diagnosis and research
Provides an update on the conventional and modern vaccines for rabies prevention
Offers updates on the full length antibodies and antibody fragments for post exposure prophylaxis of rabies
Presents technique descriptions that can be used to be compared to industry protocols to identify and establish potential new techniques

Produktinformation

Utgivningsdatum:2015-02-10
Mått:152 x 229 x 22 mm
Vikt:770 g
Format:Inbunden
Språk:Engelska
Antal sidor:384
Förlag:Elsevier Science
ISBN:9780128019191

Utforska kategorier

Biologi inom Naturvetenskap och teknik

Mer om författaren

Professor Charles E. Rupprecht is the Director of Research at the Global Alliance for Rabies Control and is the previous Director of WHO Collaborating Center for Rabies Research. He has won numerous federal service awards including the National Center for Infectious Diseases Public Health Protection Research Award in 2005. Professor Nagarajan Thirumeni is a professor in biotechnology and the manager of Indian Immunologics Ltd. as well as the general manager of research and development in the biologics division of E. Ltd, Hyderabad, India.

Recensioner i media

"...a great, up-to-date reference on laboratory protocols for rabies diagnosis, research, and prevention. It is a must if you work in this field. Score: 85 - 3 Stars" --Doody's

Innehållsförteckning

I. ForewordII. IntroductionIII. Rabies Diagnosisa) Demonstration of virusi) Electron microscopyii) Virus isolation in animals, the mouse inoculation test (MIT)iii) Virus isolation in cell culture, the rapid tissue culture inoculation test (RTCIT)iv) Rat basophilic leukemia cell sensorb) Demonstration of viral subunits and antigensi) Purification of rabies viral subunitsii) Preparation of fluorescent antibody conjugate in rabbitsiii) Preparation of fluorescent antibody conjugate in goatsiv) DFA (postmortem)v) Skin biopsyvi) Immuno histochemistryc) Demonstration of viral nucleic acidsi) Gel based PCRii) In situ PCRd) Demonstration of viral antibodies1. Binding Assaysi) ELISA - Traditionalii) IFAiii) Counter Immunoelectrophoresis2. Functional Assaysi) Mouse neutralization testii) RFFITiii) FAVNe) Typing/differentiation of lyssavirusesi) Antigenic typing by MAbsIV. Rabies Biologicsi) Rabies vaccines for humans or other animals1. Purified chick embryo cell vaccine2. Purified Vero Cell Rabies Vaccine3. In vivo NIH potency testing of rabies vaccines9. In vitro Potency testing of rabies vaccinesii) Rabies antibodies/fragments1. Production of polyclonal rabies immune globulin in humans2. Production of polyclonal antibodies in horses3. Production of polyclonal antibodies in birds4. Production of full length human monoclonal antibodies using transgenic miceV. Development of Anti-Viral ApproachesVI. Appendixi. List of biologics recommended by WHO for human rabies prophylaxis