Tumor Immunology and Immunotherapy - Cellular Methods Part B

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

• 1. Quantitation of calreticulin exposure associated with immunogenic cell deathPeng Liu, Liwei Zhao, Oliver Kepp and Guido Kroemer2. Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell deathLenka Kasikova, Iva Truxova, Isabelle Cremer, Catherine Sautes-Fridman, Oliver Kepp, Guido Kroemer, Radek Spisek and Jitka Fucikova3. Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometryGiulia Cerrato, Peng Liu, Isabelle Martins, Oliver Kepp and Guido Kroemer4. Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodiesMartina Musella, Gwenola Manic, Claudia Galassi, Ilio Vitale and Antonella Sistigu5. Methods to assess DC-dependent priming of T cell responses by dying cellsAnne-Marie Chalupova, Sarka Vosahlikova, Daniela Rozkova, Klara Sochorova, Lenka Palova-Jelinkova, Jirina Bartunkova, Radek Spisek and Jitka Fucikova6. Isolation of mononuclear phagocytes from the mouse gutPailin Chiaranunt, Kyle Burrows, Louis Ngai and Arthur Mortha7. Generation of murine bone marrow-derived macrophages and using tumor coculture assays to mimic tumor microenvironmentEsra Güç, Demi Brownlie, Carolina Rodriguez Tirado, Takanori Kitamura and Jeffrey W. Pollard8. Methods for macrophage differentiation and in vitro generation of human tumor associated-like macrophagesEsther Heideveld, Marta Horcas-Lopez, Martha Lopez-Yrigoyen, Lesley M. Forrester, Luca Cassetta and Jeffrey W. Pollard9. In vitro assessment of cancer cell-induced polarization of macrophagesDanique E.M. Duits, Max D. Wellenstein and Karin E. de Visser10. Protocol to assess the suppression of T-cell proliferation by human MDSCAnnika M. Bruger, Christophe Vanhaver, Kirsten Bruderek, Giada Amodio, Ece Tavukçuoğlu, Günes Esendagli, Silvia Gregori, Sven Brandau and Pierre van der Bruggen11. Detection and functional evaluation of arginase-1 isolated from human PMNs and murine MDSCStefania Canè and Vincenzo Bronte12. Functional assay to assess T-cell inhibitory properties of myeloid derived suppressor cells (MDSCs) isolated from the tumor microenvironment of murine glioma modelsMahmoud S. Alghamri, Neha Kamran, Padma Kadiyala, Pedro R. Lowenstein and Maria G. Castro13. FACS isolation and analysis of human circulating and tumor neutrophilsAgnieszka Swierczak and Jeffrey W. Pollard14. Flow cytometry-based isolation of tumor-associated regulatory T cells and assessment of their suppressive potentialKevin Kos, Martijn van Baalen, Denize A. Meijer and Karin E. de Visser15. Assessment of lipid load in tumor-infiltrating Tregs by flow cytometryIlenia Pacella, Alfonso Grimaldi and Silvia Piconese16. Preparation of single cells from tumors for single-cell RNA sequencingYonina Bykov, Sarah H. Kim and Dmitriy Zamarin17. Characterization of the tumor immune infiltrate by multiparametric flow cytometry and unbiased high-dimensional data analysisRomina E. Araya and Romina S. Goldszmid18. Phenotyping of tumor infiltrating immune cells using mass-cytometry (CyTOF)Pauline Maby, Aurélien Corneau and Jérôme Galon19. Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon systemMaria Belen Garcia-Fabiani, Andrea Comba, Padma Kadiyala, Santiago Haase, Felipe Javier Núñez, David Altshuler, Pedro Ricardo Lowenstein and Maria Graciela Castro20. Analysis of the immune status from peripheral whole blood with a single-tube multicolor flow cytometry assayAnna-Jasmina Donaubauer, Ina Becker, Paul F. Rühle, Rainer Fietkau, Udo S. Gaipl and Benjamin Frey21. Ex vivo assessment of in vivo DC-targeted antibodies in pre-clinical modelsQingrong Huang and Niroshana Anandasabapathy22. Flow cytometry-based assessment of direct-targeting anti-cancer antibody immune effector functionsMichelle L. Miller and Olivera J. Finn23. Enzyme-linked immunospot assay to monitor antigen-specific cellular immune responses in mouse tumor modelsDonatella Negri, Paola Sestili, Martina Borghi, Maria Ciccolella and Laura Bracci24. High-throughput analysis of cell-cell crosstalk in ad hoc designed microfluidic chips for oncoimmunology applicationsArianna Mencattini, Adele De Ninno, Jacopo Mancini, Luca Businaro, Eugenio Martinelli, Giovanna Schiavoni and Fabrizio Mattei25. Generation of affinity ranged antigen-expressing tumor cell linesAmaia Martinez-Usatorre and Pedro Romero26. Recombinant fusion proteins for targeting dendritic cell subsets in therapeutic cancer vaccineStéphanie Corgnac, Natalia K. Botelho, Alena Donda and Pedro Romero