Autophagy - From Molecular Mechanisms to Flux Control in Health and Disease

Ben Loos, Ph.D., is Professor in Physiological Sciences, Stellenbosch University, South Africa. Over the last 15 years his work has sought to better understand the role of autophagy activity, i.e. autophagic flux, in cell death onset in neurodegenerative disease and gliomas, to better quantify and control autophagy function, so as to govern cell death decision making. He focuses on the utilization of various microscopy techniques, to allow single cell and tissue analysis with high localization precision and quantitative power across scale and modality.Daniel Klionsky, PhD, is a Research Professor and Alexander G. Ruthven Professor in the Life Sciences and the Department of Molecular, Cellular and Developmental Biology, University of Michigan, USA. In 2003, he received the National Science Foundation Director's Award for Distinguished Teaching Scholars, in 2009, was elected a Fellow of the AAAS, was named a Thompson Reuters Citation Laureate in 2013, was awarded the University of Utrecht van Deenen Medal in 2015 and became a member of the American Academy of Arts and Sciences in 2019. Dr. Klionsky's lab studies the mechanism and regulation of autophagy in yeast.

• Chapter 1. How to Learn, and Teach, Autophagy.- Chapter 2. Discerning autophagy pathway intermediates – transmission electron microscopy techniques.- Chapter 3. Assessment of Autophagy: Correlative and Super-Resolution Microscopy Techniques.- Chapter 4. Autophagy receptors couple cargo destined to be degraded with the core autophagy machinery.- Chapter 5. The source of membrane during autophagy and the early steps in autophagosome formation.- Chapter 6. Autophagy and neurodegenerative diseases.- Chapter 7. Autophagy and intracellular membrane dynamics in aging and age-related diseases.- Chapter 8. Shear stress-dependent regulation of autophagy and metabolism in kidney epithelial cells.- Chapter 9. Autophagy assessment in diagnostic pathology: Focus on skeletal myopathies.- Chapter 10. The role of autophagy and mitophagy in cardiomyocyte ischemic injury.- Chapter 11. Chaperone-mediated autophagy in health and disease.- Chapter 12. A phenotypic screening routine for the identification of autophagic flux inducers.