Animal Cell Culture

"The clear and well presented protocols... are an invaluable asset...Well-presented and essential." -SGM Quarterly "The book contains 40 chapters written by a panel of authors who are all experts in their field...A wide range of topics relevant to the epidemiology, pathogenesis, clinical features and treatment of meningococcal disease are covered...this book is a valuable addition to the limited literature on meningococci and meningococcal infection. The methodological sections are detailed, carefully written and should allow technology transfer to additional laboratories." -Molecular Biotechnology

• Basic Cell Culture.- Establishment, Maintenance, and Cloning of Human Primary Cell Strains.- Aging of Cultured Human Skin Fibroblasts.- Separation and Maintenance of Primary T and B Lymphocytes.- Establishment of Lymphoblastoid Cell Lines.- Scale-Up of Suspension and Anchorage-Dependent Animal Cells.- Mycoplasma Detection.- Short-Term Chorionic Villi and Amniotic Fluid Cultures.- Keratinocyte Culture.- Establishment of Mouse Epithelial Lung Cell Strains and Cell Lines.- Culture of Human Brain Tumors on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cells and Cultured Human Glioma Cells.- Embryonic Rodent Brain Cells in Culture.- Human Thyroid Epithelial Cells.- Preparation of Isolated Rat Liver Hepatocytes.- Primary Cultures of Rat Hepatocytes.- Preparation of Isolated Periportal or Perivenous Hepatocytes from Rat Liver.- Primary Kidney Cells.- Adipocytes.- Tissue Culture of Skeletal Muscle.- Derivation and Maintenance of Embryonic Stem Cell Cultures.- Defining Hormone and Matrix Requirements for Differentiated Epithelia.- Culturing Primitive Hemopoietic Cells.- High Proliferative Potential Colony Forming Cells.- Murine Bone Marrow-Derived Macrophages.- Long-Term B Lymphoid Cultures from Murine Bone Marrow.- CFU-C in Agar.- Human Long-Term Bone Marrow Culture.- In Vitro Clonal Culture of Human Hematopoietic Progenitor Cells.- Flow Sorting for Isolating CFU-E.- Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differentiation Factors.- Clonogenic Assays for Hematopoietic and Tumor Cells Using Agar-Containing Capillaries.- Cytogenetic Techniques for Human Leukemias.- Time-Lapse Cinemicroscopy.- Transmission and Scanning Electron Microscope Preparation of Whole Cultured Cells.- Double Indirect-Immunofluorescent Labeling of Cultured Cells.- Gene Mapping to Chromosomes by Hybridization in Situ.- In Situ Hybridization with Radiolabeled cRNA Probes, Using Tissue Sections and Smears.- Immunogold Labeling for Electron Microscopy.- Human Chromosome Analysis and Sorting.- Flow Cytometry.- Fluorescence-Activated Cell Sorting According to Receptor Density.- Transfection and Transformation of Human Thyroid Epithelial Cells.- Expression of Foreign Genes in Cultured Insect Cells Using a Recombinant Baculovirus Vector.- Chloramphenicol Acetyltransferase as a Reporter in Mammalian Gene Transfer.- Immunizing Schedules for Hybridoma Production.- Fusion Protocol for the Production of Mouse Hybridomas.- Cloning of Hybridomas.- Enzyme-Linked Immunosorbent Assay for Screening of Antibodies in Hybridoma Supernatants.- Cryopreservation of Hybridomas.- Isotype Determination of Monoclonal Antibodies by Immunodiffusion.- Isoelectric Focusing and Immunofixation of Monoclonal Antibodies.- Large-Scale Production and Storage of Monoclonal Antibodies and Hybridomas.- Purification of Murine Monoclonal Antibodies.- Rat x Rat Hybridomas.- Monoclonal Antibodies Against Glycosphingolipids (GSLs)-Gangliosides.