Intestinal Stem Cells

Methods and Protocols

AvPaloma Ordóñez-Morán

Inbunden, Engelska, 2020

Del i serien Methods in Molecular Biology

1 654 kr

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Beskrivning

This detailed book encapsulates the most up-to-date methods of the intestinal stem cell field and provides guidance on a variety of techniques for studying intestinal stem cells properties.

Produktinformation

Utgivningsdatum:2020-07-24
Mått:178 x 254 x 26 mm
Vikt:889 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:350
Förlag:Springer-Verlag New York Inc.
ISBN:9781071607466

Utforska kategorier

Onkologi inom Medicin
Cellbiologi inom Naturvetenskap och teknik
Anatomi inom Medicin

Innehållsförteckning

Identification and Isolation of Human LGR5+ Cells Using an Antibody-Based Strategy.- Immune-Mediated Specific Depletion of Intestinal Stem Cells.- Analysis of Aged Dysfunctional Intestinal Stem Cells.- Strategies for Measuring Induction of Fatty Acid Oxidation in Intestinal Stem and Progenitor Cells.- Visualization of Stem Cell Niche by the Fluorescence Lifetime Imaging Microscopy.- Generation and Quantitative Imaging of Enteroid Monolayers.- Autophagy Detection in Intestinal Stem Cells.- Single-Cell Transcriptional Profiling of the Intestinal Epithelium.- Single-Cell Studies of Intestinal Stem Cell Heterogeneity during Homeostasis and Regeneration.- Large-Scale Production of Recombinant Noggin and R-Spondin1 Proteins Required for the Maintenance of Stem Cells in Intestinal Organoid Cultures.- Primary Intestinal Epithelial Organoid Culture.- In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance.- Generation of Knock-Out Gene-Edited Human Intestinal Organoids.- Direct Lineage Reprogramming of Mouse Fibroblasts to Acquire the Identity of Fetal Intestine-Derived Progenitor Cells.- Single Molecule RNA FISH in Whole-Mount Organoids.- Specific Gene Expression in Lgr5+ Stem Cells by Using Cre-Lox Recombination.- Generating and Utilizing Murine Cas9-Expressing Intestinal Organoids for Large Scale Knockout Genetic Screening.- Mouse Model for Sporadic Mutation of Target Alleles to Understand Tumor Initiation and Progression and Stem Cell Dynamics.- Hemagglutinating Virus of Japan Envelop (HVJ-E)-Guided Gene Transfer to the Intestinal Epithelium.- An Intrasplenic Injection Model for the Study of Cancer Stem Cells Seeding Capacity.- Organoid Derivation and Orthotopic Xenotransplantation for Studying Human Intestinal Stem Cell Dynamics.- Advanced Colorectal Cancer Orthotopic Patient-Derived Xenograft Models for Cancer and Stem Cell Research.- Modeling Colorectal Cancer Progression through Orthotopic Implantation of Organoids.