R.L. Phillips – författare
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2 143 kr
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Until fairly recently genetic maps of higher plants were based almost entirely on morphological and biochemical traits. These maps are rapidly being replaced and/or supplemented with DNA-based marker maps based on the use of powerful new molecular techniques. The new high-precision maps can be developed with comparative ease and rapidity. They have a much higher density of markers, which allows revelation of more and more restricted segments of the genome. One of the many revolutionary aspects of this technology is that linkage between molecular markers and traits of interest can often be detected in a single cross. The ability to hybridize probe after probe to the DNA of the same individuals of a segregating population allows one to pursue the analysis until linkage becomes evident. With morphological and biochemical markers used previously, a separate cross was required to test linkage with each new marker. It was seldom that more than three markers could be tested for linkage with the trait of interest in a single cross because of viability problems.With the new techniques described in this volume, a new gene can be placed on the linkage map within a few days instead of the much longer time required with the previous techniques. In this book, a group of leading researchers have come together to update the earlier edition of this book to include the latest versions of DNA-based marker maps for a variety of important crops. These maps illustrate the state of the art today.
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The first transgenic plants in which a bacterial gene had been stably integrated were produced in 1983, and by 1993 transgenic plants had been produced in all major crop species, including the cereals and the legumes.
Del 1 - Advances in Cellular and Molecular Biology of Plants
DNA-based markers in plants
Häftad, Engelska, 2012
541 kr
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The double helix architecture of DNA was elucidated in 1953. Twenty years later, in 1973, the discovery of restriction enzymes helped to create recombinant DNA molecules in vitro. The implications of these powerful and novel methods of molecular biology, and their potential in the genetic manipulation and improvement of microbes, plants and animals, became increasingly evident, and led to the birth of modern biotechnology. The first transgenic plants in which a bacterial gene had been stably integrated were produced in 1983, and by 1993 transgenic plants had been produced in all major crop species, including the cereals and the legumes. These remarkable achievements have resulted in the production of crops that are resistant to potent but environmentally safe herbicides, or to viral pathogens and insect pests. In other instances genes have been introduced that delay fruit ripening, or increase starch content, or cause male sterility. Most of these manipulations are based on the introduction of a single gene - generally of bacterial origin - that regulates an important monogenic trait, into the crop of choice. Many of the engineered crops are now under field trials and are expected to be commercially produced within the next few years. The early successes in plant biotechnology led to the realization that further molecular improvement of plants will require a thorough understanding of the molecular basis of plant development, and the identification and character ization of genes that regulate agronomically important multi genic traits.