Microscopy, Optical Spectroscopy, and Macroscopic Techniques

AvChristopher Jones,Barbara Mulloy

Inbunden, Engelska, 1993

Del i serien Methods in Molecular Biology

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Beskrivning

This superb survey of the possible applications of physiochemical techniques to the analysis of biological macromolecules is designed for the practicing biochemist or biologist who wants to use the technique but lacks the experience. The contributors emphasize practical aspects, such as constraints on sample quantity, purity, and presentation; the problems of time and expense involved; the problems a technique is best suited to solve; and how the results may be interpreted. Among the methods examined are optical and infrared spectroscopy, microscopy, ultracentrifugation, calorimetry, X-ray and neutron scattering, and light scattering. This book will enable the reader to confidently collaborate with specialists in applying these techniques.

Produktinformation

Utgivningsdatum:1993-11-27
Mått:155 x 235 x 15 mm
Vikt:399 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:251
Upplaga:1994
Förlag:Humana Press Inc.
ISBN:9780896032323

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Recensioner i media

...a quite fascinating book. It describes a range of techniques with which I am familiar in the literature, but have never actually used and explains the practicalities behind the instrumentation and its use...very readable and informative.-Dave Roberts, Natural History Museum-Society for General Microbiology Quarterly

Innehållsförteckning

Microscopy.- Electron Microscopy of Protein-Nucleic Acid Complexes.- Visualization of Unshadowed DNA by Electron Microscopy.- Biological Applications of Scanning Tunneling Microscopy.- Scattering And SedimentatioN.- High-Flux X-Ray and Neutron Solution Scattering.- Determination of Macromolecular Homogeneity, Shape, and Interactions Using Sedimentation Velocity Analytical Ultracentrifugation.- Determination of Absolute Molecular Weights Using Sedimentation Equilibrium Analytical Ultracentrifugation.- Classical Light Scattering for the Determination of Absolute Molecular Weights and Gross Conformation of Biological Macromolecules.- Determination of Diffusion Coefficients of Biological Macromolecules by Dynamic Light Scattering.- Calorimetric Methods.- to Microcalorimetry and Biomolecular Energetics.- Differential Scanning Calorimetry.- Isothermal Titration Microcalorimetry.- Optical Spectroscopy.- Optical Spectroscopy.- The Measurement of Electronic Absorption Spectra in the Ultraviolet and Visible.- Analysis of Polypeptide and Protein Structures Using Fourier Transform Infrared Spectroscopy.- Fluorescence Spectroscopy.- Circular Dichroism.