New Nucleic Acid Techniques

AvJohn M. Walker

Inbunden, Engelska, 1988

Del i serien Methods in Molecular Biology

2 200 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

Fler format och utgåvor

Häftad

2 202 kr

Produktinformation

Utgivningsdatum:1988-08-31
Mått:155 x 235 x 32 mm
Vikt:1 216 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:560
Upplaga:1988
Förlag:Humana Press Inc.
ISBN:9780896031272

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde.- Northern Blotting.- Hybrid-Release Translation.- Hybrid-Arrested Translation.- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells.- Use of Elutips to Purify DNA.- Plasmid Preparation on Sephacryl S1000.- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels.- Use of 35S Nucleotides for DNA Sequencing.- Direct Dideoxy DNA Sequencing.- Computer Applications to Molecular Biology.- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique.- Oligonucleotide Synthesis Using the Manual Phosphotriester Method.- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method.- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis.- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries.- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors.- Cosmid Library Construction.- Construction of cDNA Libraries in ?gt10 or ?gt11.- Immunoscreening of ?gt11 Expression Libraries Using an Avidin–Biotin Detection System.- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides.- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors.- Expression Screening of cDNA Libraries in pEX.- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins.- Epitope Mapping Using pEX.- DNA Transfection of Mammalian Cells Using Polybrene.- Expression of Foreign Genes in Mammalian Cells.- Dot-Blot Hybridization Method.- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes.- Photobiotin-Labeled DNA and RNA Hybridization Probes.- Enzyme-Labeled DNA Probes.-Luminescent Detection of Specific DNA Sequences.- Detection of DNA Sequences Using Biotinylated Probes.- Crosslinking of Single-Stranded DNA to Resins.- Extraction of RNA from Plants.- Isolation of Plant Nuclei.- In Vitro Transcription in Plant Nuclei.- Large-Scale Isolation of Ti Plasmid DNA.- Transformation and Regeneration of Dicotyledonous Plants.- Plant Protoplast Fusion.- Plant Tissue Culture.- Direct Gene Transfer into Plant Protoplasts.- Isolation of Wheat Chloroplasts and Chloroplast DNA.- DNA Labeling by Isolated Wheat Chloroplasts.

Mer från samma författare

Techniques in Molecular Biology

John M. Walker, Wim Gaastra

Häftad, 1987

555 kr

Proteins

John M. Walker

Inbunden, 1984

1 104 kr

Nucleic Acids

John M. Walker

Inbunden, 1984

1 136 kr

Proteins

John M. Walker

Häftad, 1984

1 104 kr

Nucleic Acids

John M. Walker

Häftad, 1984

1 104 kr

New Protein Techniques

John M. Walker

Inbunden, 1988

1 104 kr

Animal Cell Culture

Jeffrey W. Pollard, John M. Walker

Inbunden, 1990

2 200 kr

Plant Cell and Tissue Culture

Jeffrey W. Pollard, Jeffrey W. Pollard, John M. Walker

Inbunden, 1990

2 419 kr

Basic Protein and Peptide Protocols

John M. Walker

Inbunden, 1994

1 652 kr

Basic Protein and Peptide Protocols

John M. Walker

Häftad, 1994

1 652 kr

Mer från samma serie

Del 2834

Computational Toxicology

Orazio Nicolotti

Inbunden, 2024

2 040 kr

Del 2996

Biofuels from Algae

Kristian Spilling

Inbunden, 2026

2 200 kr

Adenovirus

Miguel Chillón, Assumpció Bosch

Häftad, 2016

1 194 kr

Ctenophores

Leonid L. Moroz

Häftad, 2025

1 981 kr

Del 2588

Oral Biology

Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng, Paul R. Cooper

Häftad, 2023

2 266 kr

Proteins

John M. Walker

Inbunden, 1984

1 104 kr

Nucleic Acids

John M. Walker

Inbunden, 1984

1 136 kr

Proteins

John M. Walker

Häftad, 1984

1 104 kr

Nucleic Acids

John M. Walker

Häftad, 1984

1 104 kr

New Protein Techniques

John M. Walker

Inbunden, 1988

1 104 kr

Du kanske också är intresserad av

10% rabatt på allt

L'amour toujours : Berättelsen om Tidö

Maggie Strömberg, Torbjörn Nilsson

Inbunden, 2026

275 kr

Plant Cell and Tissue Culture

John M. Walker, Jeffrey W. Pollard

E-bok

2008

3 054 kr

Proteins

John M. Walker

Inbunden, 1984

1 104 kr

Protein Protocols Handbook

John M. Walker

E-bok

2007

1 148 kr

Nucleic Acids

John M. Walker

E-bok

2008

1 427 kr

Techniques in Molecular Biology

Wim Gaastra, John M. Walker

E-bok

2012

718 kr

Protein Protocols Handbook

John M. Walker

3 890 kr

Animal Cell Culture

Jeffrey W. Pollard, John M. Walker

Inbunden, 1990

2 200 kr

Animal Cell Culture

John M. Walker, Jeffrey W. Pollard

E-bok

2008

2 862 kr

Proteins

John M. Walker

Häftad, 1984

1 104 kr

New Nucleic Acid Techniques

AvJohn M. Walker

Inbunden, Engelska, 1988

Del i serien Methods in Molecular Biology

2 200 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

Fler format och utgåvor

Häftad

2 202 kr

Produktinformation

Utgivningsdatum:1988-08-31
Mått:155 x 235 x 32 mm
Vikt:1 216 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:560
Upplaga:1988
Förlag:Humana Press Inc.
ISBN:9780896031272

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde.- Northern Blotting.- Hybrid-Release Translation.- Hybrid-Arrested Translation.- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells.- Use of Elutips to Purify DNA.- Plasmid Preparation on Sephacryl S1000.- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels.- Use of 35S Nucleotides for DNA Sequencing.- Direct Dideoxy DNA Sequencing.- Computer Applications to Molecular Biology.- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique.- Oligonucleotide Synthesis Using the Manual Phosphotriester Method.- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method.- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis.- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries.- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors.- Cosmid Library Construction.- Construction of cDNA Libraries in ?gt10 or ?gt11.- Immunoscreening of ?gt11 Expression Libraries Using an Avidin–Biotin Detection System.- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides.- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors.- Expression Screening of cDNA Libraries in pEX.- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins.- Epitope Mapping Using pEX.- DNA Transfection of Mammalian Cells Using Polybrene.- Expression of Foreign Genes in Mammalian Cells.- Dot-Blot Hybridization Method.- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes.- Photobiotin-Labeled DNA and RNA Hybridization Probes.- Enzyme-Labeled DNA Probes.-Luminescent Detection of Specific DNA Sequences.- Detection of DNA Sequences Using Biotinylated Probes.- Crosslinking of Single-Stranded DNA to Resins.- Extraction of RNA from Plants.- Isolation of Plant Nuclei.- In Vitro Transcription in Plant Nuclei.- Large-Scale Isolation of Ti Plasmid DNA.- Transformation and Regeneration of Dicotyledonous Plants.- Plant Protoplast Fusion.- Plant Tissue Culture.- Direct Gene Transfer into Plant Protoplasts.- Isolation of Wheat Chloroplasts and Chloroplast DNA.- DNA Labeling by Isolated Wheat Chloroplasts.