Molecular Dermatology

Methods and Protocols

AvNatalia V. Botchkareva,Gillian E. Westgate

Häftad, Engelska, 2021

Del 2154 i serien Methods in Molecular Biology

1 323 kr

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Beskrivning

This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situ, in vitro, ex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells.

Produktinformation

Utgivningsdatum:2021-04-21
Mått:178 x 254 x 16 mm
Vikt:547 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:278
Förlag:Springer-Verlag New York Inc.
ISBN:9781071606506

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Dermatologi inom Medicin

Innehållsförteckning

Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration.- Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle.- Isolation of Epidermal and Hair Follicle Melanocytes.- Isolation and Culture of Human Skin Mast Cells.- Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines.- Purification of Extracellular Microvesicles Secreted by Dermal Fibroblasts.- A Method to Investigate the Epidermal Permeability Barrier In Vitro.- Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion.- Methods to Study Human Hair Follicle Growth Ex Vivo: Human Microdissected Hair Follicle and Human Full Thickness Skin Organ Culture.- Non-Surgical Induction of Alopecia Areata in C3H/HeJ Mice via Adoptive Transfer of Cultured Lymphoid Cells.- Collapse and Restoration of Hair Follicle ImmunePrivilege Ex Vivo: A Model for Alopecia Areata.- Experimentally-Induced Epithelial-Mesenchymal Transition of Human Hair Follicle Stem Cells as a Model of Scarring Alopecia Ex Vivo.- Generation of Hair Follicle Germs in Vitro Using Human Postnatal Skin Cells.- Method for Human Eccrine Sweat Gland Isolation from the Scalp by Means of the Micro-Punch Technique and Vital Dyes.- Identification of Long Non-Coding RNA by In Situ Hybridization Approaches.- Detection of MicroRNAs by In Situ Hybridization in Skin.- Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells.- 3D-FISH Analysis of the Spatial Genome Organization in Skin Cells In Situ.- Method to Study Skin Cancer: Two-Stage Chemically-Induced Carcinogenesis in Mouse Skin.- Investigation of Skin Wound Healing Using a Mouse Model.- Human Wound Healing Ex Vivo Model with Focus on Molecular Markers.- Method for Investigation of Photobiological Effects of Light on Human Skin Cells Mediated by Low Doses of Light.