This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situ, in vitro, ex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.

This second edition volume expands on the previous edition with a discussion on the latest advancements in laboratory techniques used in dermatological research. The chapters in this book cover topics such as methodologies for establishing 2D and 3D skin models; assessing DNA damage; genome editing using CRISPR/Cas systems; analyzing extracellular vesicles involved in intercellular communication; and detecting microflora and skin permeability barrier. This book also presents experimental strategies for modeling and modulating cutaneous pathologies, including wound healing and its associated microbiome, skin cancer, and hair follicle disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Molecular Dermatology: Methods and Protocols, Second Editon is a valuable resource for researchers working in both fundamental and applied dermatological science.

This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situ, in vitro, ex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.

This second edition volume expands on the previous edition with a discussion on the latest advancements in laboratory techniques used in dermatological research. The chapters in this book cover topics such as methodologies for establishing 2D and 3D skin models; assessing DNA damage; genome editing using CRISPR/Cas systems; analyzing extracellular vesicles involved in intercellular communication; and detecting microflora and skin permeability barrier. This book also presents experimental strategies for modeling and modulating cutaneous pathologies, including wound healing and its associated microbiome, skin cancer, and hair follicle disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Molecular Dermatology: Methods and Protocols, Second Editon is a valuable resource for researchers working in both fundamental and applied dermatological science.