P2X7 Receptor

Methods and Protocols

AvAnnette Nicke

Inbunden, Engelska, 2022

Del i serien Methods in Molecular Biology

2 406 kr

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Beskrivning

This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models.

Produktinformation

Utgivningsdatum:2022-07-02
Mått:178 x 254 x 28 mm
Vikt:961 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:394
Förlag:Springer-Verlag New York Inc.
ISBN:9781071623831

Utforska kategorier

Innehållsförteckning

How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating.- Agonists, Antagonists, and Modulators of P2X7 Receptors.- Modulation of Cell Energy Metabolism by the P2X7 Receptor.- Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE.- Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4).- Development of Antibody and Nanobody Tools for P2X7.- Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors.- Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations.- Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes.- A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors.- Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents.- Using SymmetricalOrganic Cation Solutions to Study P2X7 Ion Permeation.- Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors.- Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry.- Characterization of P2X7 Receptors in Human Blood Cells.- Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in Multiple Cell Populations Mixed in a Single FACS Tube.- Administration of P2X7 Receptor Blockers in Oncological Experimental Models.- Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease.- Analysis of P2X7-Induced Neuronal Branching.- Studying the Role of P2X7 Receptor in Axonal Growth Using In Utero Electroporation Technique.- Analyzing the Role of the P2X7 Receptor in Epilepsy.