Plant Developmental Biology

Methods and Protocols

AvLars Hennig,Claudia Köhler

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

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Beskrivning

Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 25 mm
Vikt:858 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:448
Förlag:Humana Press Inc.
ISBN:9781493957347

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

Growth Protocols for Model Plants in Developmental Biology.- Grafting as a Research Tool.- Virus-Induced Gene Silencing as a Reverse Genetics Tool to Study Gene Function.- The CRE/lox System as a Tool for Developmental Studies at the Cell and Tissue Level.- Inducible Gene Expression Systems for Plants.- Trichome Development in Arabidopsis.- Phenotyping the Development of Leaf Area in Arabidopsis thaliana.- Analyzing Shoot Apical Meristem Development.- Analyzing Floral Meristem Development.- Female Gametophytic Mutants: Diagnosis and Characterization.- Pollen Tube Development.- Analysis of Root Meristem Size Development.- Phenotypic Characterization of Photomorphogenic Responses During Plant Development.- Kinematic Analysis of Cell Division and Expansion.- Flowering Time Control.- mRNA Detection by Whole Mount In Situ Hybridization (WISH) or Sectioned Tissue In Situ Hybridization (SISH) in Arabidopsis.- Immunolocalization of Proteins in Plants.- Detection of Small Non-coding RNAs.- Quantitative Real Time PCR in Plant Developmental Biology.- Luciferase and Green Fluorescent Protein Reporter Genes as Tools to Determine Protein Abundance and Intracellular Dynamics.- Fluorescence-Activated Cell Sorting in Plant Developmental Biology.- Laser Microdissection of Paraffin-Embedded Plant Tissues for Transcript Profiling.- Utilizing Bimolecular Fluorescence Complementation (BiFC) to Assay Protein–Protein Interaction in Plants.- The Split Luciferase Complementation Assay.- Co-immunoprecipitation and Protein Blots.- Probing Protein–Protein Interactions with FRET–FLIM.- Plant Chromatin Immunoprecipitation.- Immunocytological Analysis of Chromatin in Isolated Nuclei.- Bisulphite Sequencing of Plant Genomic DNA.