Heterologous Expression of Membrane Proteins

Methods and Protocols

AvIsabelle Mus-Veteau

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

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Beskrivning

This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs).

Produktinformation

Utgivningsdatum:2018-05-30
Mått:178 x 254 x 25 mm
Vikt:666 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:322
Upplaga:2
Förlag:Humana Press Inc.
ISBN:9781493981021

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functionaland Structural Studies.- Membrane Protein Production in the Yeast, S. cerevisiae.- Membrane ProteinsProduction in Escherichia coli:Protocols and Rules.- Codon Optimizing for Increased Membrane ProteinProduction: A Minimalist Approach.- Generation of Tetracycline-InducibleMammalian Cell Lines by Flow Cytometry for Improved Overproduction of MembraneProteins.- Membrane Protein Production in Lactococcus lactis for FunctionalStudies.- Expression ofViral Envelope Glycoproteins in Drosophila melanogaster S2 Cells.- Leishmania tarentolæ as a Promising Toolfor Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic MembraneProteins: The Case of the ABC Pump ABCG6.- Overexpression, MembranePreparation, and Purification of a Typical Multidrug ABC Transporter BmrA.- Expression ofEukaryotic Membrane Proteins in Pichiapastoris.- Integral Membrane Protein Expression in Saccharomyces cerevisiae.- High-Throughput Baculovirus ExpressionSystem for Membrane Protein Production.- Small-Scale Screening to Large-ScaleOver-Expression of Human Membrane Proteins for Structural Studies.- Purificationof Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies.- MembraneProtein Solubilization and Composition of Protein Detergent Complexes.- Detergent-FreeMembrane Protein Purification.- Conformational Dynamics and Interactions ofMembrane Proteins by Hydrogen/Deuterium Mass Spectrometry.- Lessons from an a-Helical Membrane Enzyme: Expression,Purification, and Detergent Optimization for Biophysical and StructuralCharacterization.- Method to Screen Multidrug Transport Inhibitors Using YeastOver-Expressing a Human MDR Transporter.