This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.

This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.