Atlas of Living Cell Cultures

Toni Lindl is Professor emeritus of the University of Applied Sciences in Weihenstephan, Germany, and CEO of the Institute of Applied Cell Culture Ltd., Munich. After studies of biochemistry and cell biology he obtained his PhD in neurochemistry. Following post doc fellowships at the University of Constance and Bonn, Germany, he founded the Institute of Applied Cell Culture Ltd. in 1981 and in 1990 received a professorship at the University of Applied Sciences in Weihenstephan. He is author of the first German textbook in cell and tissue culture (currently in its 7th Edition) and wrote more than 70 articles in many prestigious journals, among those are Nature, BBA, ALTEX etc. He is member of several cell biology societies and is engaged in the development of alternatives to animal testing (3R according to Russell and Burch).Rosemarie Steubing is head of the cell culture company CLS Cell Lines Service GmbH since 2004. After her study of chemistry and biochemistry at the Ruprecht-Karls-University Heidelberg, Germany, she obtained her Ph.D. in applying laser microirradiation to the fusion of single cells. Following two years as post-doctoral fellow at the Beckman Laser Institute, University of California, Irvine, USA, she has been working in molecular physiology and cellular immunology. In the field of laser micromanipulation and optical trapping of single cells, of single skeletal muscle fibers and motility assays using purified actin and myosin, she has authored several scientific publications and book chapters.

• Preface and Acknowledgments VIIAbbreviations XI1 Introduction 11.1 Introduction and Usage of This Book 11.2 General Remarks 22 Basic Cell Culture Techniques 52.1 Safety Precautions for Frozen Cell Lines 52.2 Sterile Working 52.3 Handling Procedure for Cell Lines 52.3.1 Frozen Cells 52.3.2 Receipt of Growing Adherent Cultures in T-flasks 62.3.3 Receipt of Growing Suspension Cultures 62.3.4 Medium Replacement of Cells in Suspension 62.3.5 Subculture of Cells in Suspension 72.3.6 Subculture of Adherent Cells 72.3.7 Subculture of Mixed Cell Lines (Adherent and Floating Cells) 72.3.8 Cell Counting 72.3.9 Cryopreservation of Cell Lines 72.3.10 Long Term Storage of Cells 82.3.11 Detection and Elimination of Contaminations 82.3.12 Cross-contaminations=Authentication 92.4 Special Remarks on the Origin of the Cell Lines 92.5 Photographic Equipment 93 List of Cell Lines and Human Primary Cells (in Alphabetical Order) 113.1 Human Cell Lines 113.2 Animal Cell Lines 123.2.1 Rat 123.2.2 Mouse 123.2.3 Hamster 133.2.4 Chicken 133.2.5 Monkey 133.2.6 Pig 133.2.7 Opossum 133.2.8 Potoroo 133.2.9 Bovine 133.2.10 Dog 143.2.11 Insect 143.3 Human Primary Cells 144 Cell Lines and Human Primary Cells 154.1 Human Cell Lines 154.2 Animal Cell Lines 3414.2.1 Rat 3414.2.2 Mouse 3674.2.3 Hamster 4334.2.4 Chicken 4374.2.5 Monkey 4434.2.6 Pig 4514.2.7 Opossum 4574.2.8 Potoroo 4614.2.9 Bovine 4674.2.10 Dog 4714.2.11 Insect 4754.3 Human Primary Cells 479Appendix A: Materials and Suppliers 493Appendix B: Suppliers of Cell Culture Materials 497Further Reading 501Index 503