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Beskrivning
This updated and extended second edition of the textbook introduces the basic concepts of bioinformatics and enhances students' skills in the use of software and tools relevant to microbiology research.
Henrik Christensen (MSc, Ph.D., DVSc) is an Associate Professor specializing in population genomics and bioinformatics at the Department of Veterinary Animal Science, University of Copenhagen. He investigates the transmission of opportunistic pathogens, including serotype prediction, and studies the taxonomy of members of Pasteurellaceae. He is in charge of a Ph.D. course in bioinformatics focusing on microbiology with ad hoc expertise in the analysis of avian pathogenic Escherichia coli.
Innehållsförteckning
1. Introduction.- 2. DNA sequence asssembly and annotation of genes.- 3. Databases and protein structures.- 4. Pairwise alignment, multiple alignment and BLAST.- 5. Primer design.- 6. Introduction to phylogenetic analysis of molecular sequence data.- 7. Sequence based classification and identification.- 8. 16S rRNA amplicon sequencing.- 9. Full shot-gun DNA metagenomics.- 10. Transcriptomics.- 11. Sequenced based typing and prediction of function.