Peroxisome Proliferator-Activated Receptors (PPARs)

Methods and Protocols

AvMostafa Z. Badr,Jihan A. Youssef

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

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Beskrivning

All three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology but differ in function, tissue distribution and ligand specificity.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x undefined mm
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:343
Förlag:Humana Press Inc.
ISBN:9781493962570

Utforska kategorier

Innehållsförteckning

PPARs: History and Advances.- PPAR-Alpha Cloning, Expression and Characterization.- PPARGC1A and PPARG Genotyping. Beginner’s Guide to Genotyping with Unlabeled Probes.- Generation of an Inducible, Cardiomyocyte-Specific Transgenic Mouse Model with PPARβ/d Overexpression.- Specific knockdown of PPARd Gene in Colon Cancer Cells by Lentivirus-Mediated RNA Interfering.- Dominant-Negative and Knockdown Approaches to Studying PPAR activity.- Producing PPARgamma2 Knockdown in Mouse Liver.- Adipose Tissue-Specific PPARg Gene Targeting.- Site-Directed Mutagenesis to Study the Role of Specific Amino Acids in the Ligand Binding Domain of PPARs.- PPAR SUMOylation: Some Useful Experimental Tips.- Analyzing Phosphorylation-Dependent Regulation of Subcellular Transcriptional Activity of Transcriptional Coactivator NT-PGC-1a.- In vivo Studies of PPAR-Chromatin Interactions: Chromatin Immunoprecipitation for Single-Locus and Genomewide Analyses.- FISH Analysis Using PPARg Specific Probes for Detection of PAX8-PPARg Translocation in Follicular Thyroid Neoplasms.- Immunohistochemical Techniques to Identify and Localize Proteins of Interest in Paraffin-Embedded Tissue Sections.- Determination of PPAR Expression by Western-Blot.- Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators.- Estimation of the PPARa Agonism of Fibrates by a Combined MM-Docking Approach.- Combined Biophysical and Cell-based Approaches for the Assessment of Ligand Binding to PPARg.- Exploring PPAR Modulation in Experimental Mice.- Induction of Adipogenic Differentiation in Three-Dimensional Culture Model on a Novel Microfabricated Scaffold.- Analyzing PPAa/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry.- Synthesis, Mass Spectrometric Characterization, and Analysis of the PPARd Agonist GW1516 and its Major Human Metabolites – Targets in Sports Drug Testing.- LC-MS-Based Method for the Qualitativeand Quantitative Analysis of the Novel PPARg Agonist KR-62980.- Behavioral Paradigms to Evaluate PPAR Modulation in Animal Models of Brain Injury.