Hydrocarbon and Lipid Microbiology Protocols

Ultrastructure and Imaging

AvTerry J. McGenity,Kenneth N. Timmis

Häftad, Engelska, 2018

Del i serien Springer Protocols Handbooks

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Beskrivning

This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells.

Produktinformation

Utgivningsdatum:2018-04-22
Mått:178 x 254 x 11 mm
Vikt:358 g
Format:Häftad
Språk:Engelska
Serie:Springer Protocols Handbooks
Antal sidor:174
Förlag:Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
ISBN:9783662569832

Utforska kategorier

Patologi inom Medicin
Biologi inom Naturvetenskap och teknik
Tillverkningsteknik inom Naturvetenskap och teknik

Innehållsförteckning

Introduction.- Electron microscopy protocols for the study of hydrocarbon-producing and -decomposing microbes: classical and advanced methods.- Protocol for laser scanning microscopy of microorganisms on hydrocarbons.- Fluorescence microscopy for microbiology.- Imaging bacterial cells and biofilms adhering to hydrophobic organic compounds-water interfaces.- Bacteria-mineral colloid interactions in biofilms - an ultrastructural and microanalytical approach.- Identification of microorganisms in hydrocarbon contaminated aquifer samples by fluorescence in-situ hybridization (CARD-FISH).- Studies of the ecophysiology of single cells in microbial communities by (quantitative) microautoradiography and fluorescence in situ hybridization (MAR-FISH).- Protocol for in situ detection of functional genes of microorganisms by Two-pass TSA-FISH.- Three-dimensional visualization and quantification of lipids in microalgae using confocal laser scanning microscopy.- A Correlative Light-Electron Microscopy (CLEM) protocol for the identification of bacteria in animal tissue, exemplified by methanotrophic symbionts of deep-sea mussels.