Circadian Rhythms and Biological Clocks Part A

Dr. Sehgal is the John Herr Musser Professor of Neuroscience and Director of a Program in Chronobiology at the Perelman School of Medicine, University of Pennsylvania. She has also been an Investigator of the Howard Hughes Medical Institute since 1997. Dr. Sehgal received her Ph.D. from the Graduate School of Medical Sciences, Cornell University, working with Dr. Moses Chao, and conducted her postdoctoral work with Dr. Michael Young at Rockefeller University. A major focus in her laboratory is the cellular and molecular basis of circadian rhythms, using the fruit fly, Drosophila melanogaster as a model system. Her laboratory also developed a Drosophila model for the study of sleep, which has been adopted by laboratories worldwide, and is rapidly provding insight into the regulation and function of sleep. In 2009, Dr. Sehgal was elected to the Institute of Medicine and in 2011 to the American Academy of Arts and Sciences.

"…these volumes provide a remarkable summary of the methods, experimental systems, and findings of cutting-edge research into circadian rhythms….an essential resource for all types of scientists studying chronobiology, as well as an informative read for anyone who wishes to understand better the ticking of their biological clock." --The Quarterly Review of BiologyPraise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia"The Methods in Enzymology series represents the gold-standard." --Neuroscience

• 1. Studying Circadian Rhythm and Sleep Using Genetic Screens in Drosophila Sofia Axelrod, Lino Saez and Michael W. Young2. Dissecting the Mechanisms of the Clock in NeurosporaJennifer Hurley, Jennifer J. Loros and Jay C. Dunlap3. High Throughput and Quantitative Approaches for Measuring Circadian Rhythms in Cyanobacteria Using BioluminescenceRyan K. Shultzaberger, Mark L. Paddock, Takeo Katsuki, Ralph J. Greenspan and Susan S. Golden4. Using Circadian Entrainment to Find Cryptic ClocksZheng Eelderink-Chen, Maria Olmedo, Jasper Bosman and Martha Merrow5. Wavelet-based Analysis of Circ adian Behavioral RhythmsTanya L. Leise6. Genetic Analysis of Drosophila Circadian Behavior in Semi-Natural ConditionsEdward W Green, Emma K O’Callaghan, Mirko Pegoraro, J Douglas Armstrong, Rodolfo Costa3 and Charalambos P Kyriacou7. Methods to Study Molecular Mechanisms of the Neurospora Circadian ClockJoonseok Cha, Mian Zhou, and Yi Liu8. Detecting KaiC Phosphorylation Rhythms of the Cyanobacterial Circadian Oscillator in Vitro and in VivoYong-Ick Kim, Joseph S. Boyd, Javier Espinosa, and Susan S. Golden9. The Role of Casein Kinase I in the Drosophila Circadian ClockJeffrey L. Price, Jin-Yuan Fan, Andrew Keightley and John C. Means10. Purification and Analysis of PERIOD Protein Complexes of the Mammalian Circadian ClockJin Young Kim, Pieter Bas Kwak, Michael Gebert, Hao A. Duong and Charles J. Weitz11. Best Practices for Fluorescence Microscopy of the Cyanobacterial Circadian ClockSusan E. Cohen, Marcella L. Erb, Joe Pogliano and Susan S. Golden12. Structural and Biophysical Methods to Analyze Clock Function and Mechanism Martin Egli13. Identification of Small Molecule Modulators of the Circadian ClockTsuyoshi Hirota and Steve A. Kay14. ChIP-Seq and RNA-Seq Methods to Study Circadian Control of Transcription in MammalsJoseph S. Takahashi, Vivek Kumar , Prachi Nakashe, Nobuya Koike, Hung-Chung Huang, Carla B. Green, and Tae-Kyung Kim 15. Chipping away at the Drosophila ClockJian Zhou, Wangjie Yu and Paul E. Hardin16. Considerations for RNA-seq Analysis of Circadian RhythmsJiajia Li, Gregory R Grant, John B Hogenesch and Michael E Hughes17. RNA-Seq Profiling of Small Numbers of Drosophila NeuronsKatharine Abruzzi, Xiao Chen, Emi Nagoshi, Abby Zadina, and Michael Rosbash18. Analysis of Circadian Regulation of Poly(A) Tail Length Shihoko Kojima and Carla B. Green19. Sample Preparation for Phosphoproteomic Analysis of Circadian Time Series in Arabidopsis ThalianaJohanna Krahmer, Matthew M Hindle, Sarah F Martin, Thierry Le Bihan and Andrew J Millar