Immuno-oncology and immunotherapy Part A

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Norma received her master’s degree in health biology from Paris Saclay. In 2013, she joined the laboratory of Dr. Guido Kroemer at the Centre de Recherche des Cordeliers (Paris, France) and at Gustave Roussy (Villejuif, France), the largest center for oncological patients in Europe. She worked on several projects linked to immunosurveillance, culminating with her obtaining her PhD in 2017 with an original work on “Immunogenic stress and death of cancer cells: Contribution of antigenicity vs adjuvanticity to immunosurveillance”. She then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Galluzzi. Her current research is focused on investigating resistance to immunotherapy in a mouse model of HR+ breast cancer.Maud Charpentier received her M.Sc. in Cellular Biology and her Ph.D. in Immunology from the University of Nantes, France. She chose to pursue an academic career and continued her postdoctoral training in the United States. She joined the Department of Radiation Oncology at Weill Cornell Medicine under the mentorship of Dr. Sandra Demaria. Maud has a long-standing interest in the anti-tumor immune response and its role in controlling cancer progression and treatment outcomes. Her research focuses on understanding the synergy between radiation therapy and immunotherapy in solid tumors, with the aim of overcoming resistance to treatment and developing innovative therapeutic approaches in preclinical models.

• 1. Assessment of translation rate in leukemic cells and immune cells of the microenvironment by OPP protein synthesis assay Vanessa Klapp, Ozgu Gumustekin, Jérôme Paggetti, Etienne Moussay and Anne Largeot 2. Generation of transmitochondrial cybrids in cancer cells Ruth Soler-Agesta, Cristina Ripollés-Yuba, Joaquín Marco-Brualla, Raquel Moreno-Loshuertos, Ai Sato, Manuel Beltrán-Visiedo, Lorenzo Galluzzi and Alberto Anel 3. Enzyme-linked Immunospot (Elispot) assay to quantify peptide-specific IFN-γ production by splenocytes in a mouse tumor model after radiation therapy Benoît Lecoester, Yaoyao Xie, Amélie Marguier, Laura Boulerot, Marine Malfroy, Olivier Adotévi and Jihane Boustani 4. Tumor slice culture system for ex vivo immunotherapy studies Leire Arrizabalaga, Joan Salvador Russo-Cabrera, Virginia Belsúe, Pedro Berraondo, Ignacio Melero and Fernando Aranda 5. Identification and analysis of alloreactive T lymphocytes from peripheral blood mononuclear cells Alberto Susana, Giovanni Galletti, Gabriele De Simone, Chiara Camisaschi and Enrico Lugli 6. Assessment of pDCs functional capacity upon exposure to tumor-derived soluble factors Vladimír Koucký, Linn A. Syding, Klára Plačková, Lucie Pavelková and Anna Fialová 7. Degranulation assay to evaluate NK cell natural and antibody-dependent cell-mediated cytotoxicity against A549 tumor spheroids Ainara Lopez-Pardo, Ainhoa Amarilla-Irusta, Víctor Sandá, Mario Stan, Francisco Borrego and Laura Amo 8. Characterization of Natural Killer cells infiltrating irradiated murine tumors through flow cytometry Jérémy Baude, Riad Ladjohounlou, Emeric Limagne, Lisa Froidurot, Véronique Morgand and Céline Mirjolet 9. Flow cytometry detection and quantification of circulating leukocyte subpopulations in mice after brain irradiation Julie Coupey, Marine M. Leblond, Erika S. Hue and Samuel Valable 10. Flow cytometry-assisted analysis of phenotypic maturation markers on an immortalized dendritic cell line Ginevra Campia, Manuel Beltrán-Visiedo, Ruth Soler-Agesta, Ai Sato, Norma Bloy, Liwei Zhao, Peng Liu, Oliver Kepp, Guido Kroemer, Lorenzo Galluzzi and Claudia Galassi 11. Optimization and intra-assay validation of a multiparametric flow cytometric test for monitoring circulating TREGs Iole Macchia, Floriana Iacobone and Francesca Urbani 12. Cytofluorometric assessment of calreticulin exposure on CD38+ plasma cells from the human bone marrow Manuel Beltrán-Visiedo, Alfonso Serrano-Del Valle, Nelia Jiménez-Aldúan, Ruth Soler-Agesta, Javier Naval, Lorenzo Galluzzi and Isabel Marzo