Protein Purification

Principles, High Resolution Methods, and Applications

AvJan-Christer Janson

Inbunden, Engelska, 2011

Del 149 i serien Methods of Biochemical Analysis

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Beskrivning

The authoritative guide on protein purification—now completely updated and revisedSince the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: High-resolution reversed-phase liquid chromatography Electrophoresis in gels Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients Affinity ligands from chemical and biological combinatorial libraries Membrane separations Refolding of inclusion body proteins from E. coli Purification of PEGylated proteins High throughput screening techniques in protein purification The history of protein chromatography

Produktinformation

Utgivningsdatum:2011-04-08
Mått:224 x 292 x 38 mm
Vikt:1 497 g
Format:Inbunden
Språk:Engelska
Serie:Methods of Biochemical Analysis
Antal sidor:544
Upplaga:3
Förlag:John Wiley & Sons Inc
ISBN:9780471746614

Utforska kategorier

Organisk kemi inom Naturvetenskap och teknik

Mer om författaren

JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.

Innehållsförteckning

Preface to The Third Edition viiPreface to The Second Edition ixPreface to The First Edition xiContributors xiiiPart I Introduction 11 Introduction to Protein Purification 3Bo Ersson, Lars Rydén, and Jan-Christer JansonPart II Chromatography 232 Introduction to Chromatography 25Jan-Christer Janson and Jan Åke Jönsson3 Gel Filtration: Size Exclusion Chromatography 51Lars Hagel4 Ion Exchange Chromatography 93Evert Karlsson and Irwin Hirsh5 High-Resolution Reversed-Phase Chromatography of Proteins 135Sylvia Winkel Pettersson6 Hydrophobic Interaction Chromatography 165Kjell-Ove Eriksson and Makonnen Belew7 Immobilized Metal Ion Affinity Chromatography 183Lennart Kågedal8 Covalent Chromatography 203Francisco Batista-Viera, Lars Rydén, and Jan Carlsson9 Affinity Chromatography 221Francisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson10 Affinity Ligands from Chemical Combinatorial Libraries 259Enrique Carredano and Herbert Baumann11 Affinity Ligands from Biological Combinatorial Libraries 269Per- Åke NygrenPart III Other Separation Methods and Related Techniques 27912 Membrane Separations 281Joachim K. Walter, Zuwei Jin, Maik W. Jornitz, and Uwe Gottschalk13 Refolding of Inclusion Body Proteins from E. coli 319Zhiguo Su, Diannan Lu, and Zheng Liu14 Purification of PEGylated Proteins 339Conan J. Fee and James M. Van AlstinePart IV Electrophoresis 36315 Electrophoresis in Gels 365Reiner Westermeier16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients 379Pier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti17 Two-Dimensional Electrophoresis in Proteomics 411Reiner Westermeier and Angelika Görg18 Protein Elution and Blotting Techniques 441Reiner Westermeier19 Capillary Electrophoretic Separations 451Wolfgang ThormannPart V Separation Method Optimization 48720 High Throughput Screening Techniques in Protein Purification 489Karol M. Lacki and Eggert BrekkanIndex 507