Chimeric Antigen Receptor T Cells

Development and Production

AvKamilla Swiech,Kelen Cristina Ribeiro Malmegrim

Häftad, Engelska, 2020

Del 2086 i serien Methods in Molecular Biology

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Beskrivning

This volume provides comprehensive methods from expert scientists working in the Chimeric Antigen Receptor T Cell (CAR-T Cell) field. Chapters guide readers through the state-of-art of CAR-T cell technology, CAR design and vector production, CAR-T cell generation and manufacturing, CAR-T cell characterization, and quality control.

Produktinformation

Utgivningsdatum:2020-11-10
Mått:178 x 254 x 16 mm
Vikt:547 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:274
Förlag:Springer-Verlag New York Inc.
ISBN:9781071601488

Utforska kategorier

Onkologi inom Medicin
Cellbiologi inom Naturvetenskap och teknik
Farmakologi inom Medicin

Innehållsförteckning

CAR-T Cells for Cancer Treatment: Current Design and Next Frontiers.- Selection of Antibody Fragments for CAR-T Cell Therapy from Phage Display Libraries.- Phase I/II Manufacture of Lentiviral Vectors under GMP in an Academic Setting.- Production of Lentivirus for the Establishment of CAR-T Cells.- Optimized Production of Lentiviral Vectors for CAR-T Cell.- Lentiviral Vectors Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells.- In vitro Transcribed (IVT)-mRNA CAR Therapy Development.- Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors.- Generation of CAR+ T Lymphocytes using the Sleeping Beauty Transposon System.- Platforms for Clinical-grade Car-T Cell Expansion.- CAR-T Cell Expansion in a Xuri Cell Expansion System W25.- Methods and Process Optimization for Large Scale CAR T Expansion Using the G-Rex Cell Culture Platform.- Replication Competent LentivirusAnalysis of Vector Transduced T Cell Products used in Cancer Immunotherapy Clinical Trials.- Immunophenotypic Analysis of CAR-T Cells.- Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies.- Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays.- Analysis of CAR-mediated Tonic Signaling.- Generation of Tumor Cells Expressing Firefly Luciferase (fLuc) to Evaluate the Effectiveness of CAR in a Murine Model.- Analysis of Antitumor Effects of CAR-T Cells in Mice with Solid Tumors.